eseess中的snp引起的剪接方式差异的研究word格式论文.docx

下载文档 降价啦

2
0
约5.17万字
约 57页
2018-05-18 发布于上海
举报
版权申诉
保障服务

eseess中的snp引起的剪接方式差异的研究word格式论文.docx

1、本文档共57页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

eseess中的snp引起的剪接方式差异的研究word格式论文

AbstractAlternativesplicingofpre-mRNAisanimportantphenomenonexistingingenes ofeukaryote,whichplaysavitalpoleingeneexpressionregulation.Problemsabout mechanismscontrolalternativesplicingisaleadingresearchendeavorsinfunctional genome era.Individualvarianceisprevalentinsensitivitytomedical treatment.Currently screeningapproachesbasedoncandidategenemethodandwholegenomewide screeninghasprovedthereexistsgeneticbasisfordifferenceinsensitivitytodrugs. Furtherexonarrayanalysisshowedthatdifferentpopulationshaveexhibited differentialexonlevelexpressionafterspecificdrugtreatment,whichisclosed connectedwithindividualvariation.Tocomprehensivedecipherthegeneticvariations contributingtothesedifference,wefirstutilizetheexpressionprofileoftwokindsof lymphoblastoidcelllines, 30CEU(Utahresidentsfrom Centerd’Etudedu PolymorphismeHumainpopulation)and30YRI(YorubainIbadan,Nigeria),in etoposide-inducedcytotoxicity,thenFIRMA (FindingIsoformsUsingRobust MultichipAnalysis)andMIDAS(MicroarrayDetectionofAlternativeSplicing) algorithmareappliedseparatelytoinvestigateexpressiondifference,aimingto excludefalsepositivedata.Inthenextstep,SingleNucleotidePolymorphism(SNP) dataprovidedbyHapMap(HaplotypeMapofthehumangenome)projectand regulatorymotifsidentifiedthoughcomputationalapproach,wearecapableof establishtheconnectionbetweenSNP sitesanddifferentiatedsplicedexon.Finallywe haveextractedfeaturesofsplicingregulatoryelementfromexonsskippedand includedtoidentifycausativeSNPswhichcan introducedifferencegenesplicing pattern.CelllinesarederivedfromdescentsofEuropeanandAfrican,andtheexon expressionlevelaredeterminedbyAffymetrixGeneChipHumanExon1.0STArray. Inourstudy,52SNPshavebeenidentifiedtobecableofinducingexondifferential splicingbychangingthebalanceofpositiveandnegativesplicingregulatoryelements composition.TheseSNPsarelocatedexclusivelyinthetwopopulationswhichmeans eachSNPsiteexistsonlyinonepopulation.Duetolackofbiologicalevidencewhich wouldbeusedtoverifythepredictedSNPs,wehavedesignedanemulationdatase