ibpirf4结合蛋白在人涎腺腺样囊性癌中表达及功能的实验分析-experimental analysis of ibp irf 4 binding protein expression and function in human salivary adenoid cystic carcinoma.docxVIP

importantfunctions.Nevertheless,theexactrolesplayedbyIBPinSACCremainunclear.Inourstudy,forthefirsttime,weinvestigatedtherelationshipbetweenIBPexpressionandclinicopathologicalcharacteristicsinSACCandnormalsaliarygland.Furthermore,weestablishedIBP-overexpressingstablecelllineandinvestigatedtheeffectofIBPinSACCcellsinvitro.Methodsandresults：TheResearchofIBPExpressioninSACCandnormalsaliaryglandanditsClinicalSignificance①WeexaminedIBPexpressionintwoSACCcelllines,ACC2andACCM,byWesternBlot.TheresultsshowthatthereisnegativeexpressionofIBPinACC2andACCM.②Wecollected27casesofSACCparaffinspecimensand20casesofnormalsaliaryglandtissuesspecimens,throughtheimmunohistochemistytoinvestigatetheexpressionofIBPinclinicalsamples,andtodeterminetheclinicalsignificancebystatisticalanalysisbetweenIBPexpressionandotherpathologicalindexes.TheresultindicatedthatonlythreecasesofweakpositiveIBPgeneexpressionin27casesofSACC,theotherexpressnegative;IBPwasexpressedstronglyinall20casesofnormalsaliaryglandtissues,thereexistsignificantdifferences（P0.05）.ButtheexpressionofIBPwerenotsignificantlyrelatedtopatient’sage,genderandtumorsite（P0.05）.TheStudyofIBPFunctionandMechanisminSACC①TheconstructionofIBPover-expressionmodelbyplasmidtransfectionwasbasedonIBPnegativeexpressioncellline-ACC2.ItwasconfirmedthatwehadgottenstablecelllinesofIBPover-expressionbyWesternBlot.②TheMTTassaywasusedtodeterminetheeffectsofIBPoncellproliferationineachgroup.InourstudywefoundthatIBPcaninhibittheproliferationofSACCcells.③PlatecolonyformationassaywasusedtodeterminetheeffectsofIBPoncellgrowthinSACCcells.ItwasshownthatoverexpressionofIBPcaninhibittheabilityofcolonyformationofSACCcells.④Theabilityofcellinvasionwasbasedonanalysisofstained-cellspenetratingstudybyTranswellexperimentstodeterminetheeffectsofIBPontheabilityofcellinvasion;theresultshowedthatIBPcanpromoteSACCcellsinvasion.⑤ToobservetheinfluenceofIBPonSACCcellsresistingtotaxolwhichiscommonlyusedinclinicaltreatmentofSACC;theresultindicatedthatIBPcanresisttotaxoltoacertainde