Airyscan超分辨率技术在生命科学和医学中的应用.pdfVIP

ZEISS LSM 880 with Airyscan Revolutionize Your Confocal Imaging Carl Zeiss Microscopy GmbH Confocal Microscope: Working horse of imaging Huge installed base, accessible, widespread usage Imaging experiments become more intricate and challenging. The working horse of imaging must fit the task. Carl Zeiss Microscopy 2 LSM 880 brings new, better, and faster capabilities. 140 nm Carl Zeiss Microscopy 3 At the heart of the LSM: The Confocal Principle Rejection of out-of-focus light at the pinhole and 3D Imaging PMT Pinhole Confocal Plane Laser MBS 488 Objective Focal Plane Specimen Carl Zeiss Microscopy 4 Confocal Microscopy is 3D Point scanning vs. “snapshot” techniques Wide Field Excitation Emission Wide Field (laser) Plan-APOCHROMAT Plan-APOCHROMAT 40x /1,3 Oil 40x /1,3 Oil z limited z-resolution “snapshot” thick sections