(病原免疫实验课件)实验一_凝集试验知识讲稿.pptVIP

* Maybe result is like this. ------ What is negative, What are 4 plus signs, 3 plus signs,2 plus signs or 1 plus sign. * * * * * 先做类风湿因子测定 微量移液器的使用 How to Use a Micropipettor Set the desired volume by turning the centrally located rings. (Never exceed the upper or lower limits of pipettors. ) (20/40ul~200ul) Place a tip on the discharge end of the pipettor. Depress the plunger until you feel the initial resistance and insert tip into the solution, just barely below the surface of the liquid and not as deep as possible.(for accurate/precise volume) Carefully and slowly release plunger. (Allow the pipet tip to fill to final volume before removing it from solution to avoid the presence of bubbles in the plastic tip.) Discharge the solution into the appropriate container by depressing plunger. Remember to change tips between different solutions to avoid mixing or contaminating the solutions used. （一）3、协同凝集试验 金葡菌 SPA 已知抗体 （ 致敏金葡菌 ） Ag ＋ 金葡菌－Ab → 凝集块(协同凝集反应) 请按照实验指导讲义方法进行操作； 注意观察第1格内的凝集现象，并分析第2、3格为何不出现凝集。 1. Ab- s.aureus Ab- s.aureus s.aureus 2. CSF(Ag) normal saline CSF(Ag) 3. joggle, 4. (result) agglutinate × × CSF: cerebrospinal fluid from the patient with meningitis, There are meningococcuses(Ag) in CSF from the patient with bacterial meningitis. (two persons each group) 实验报告： 一、原理 二、现象 三、结论 （二）非特异性免疫实验 ───吞噬试验（示教） 1、中性粒细胞的吞噬作用（小吞噬） 了解小吞噬试验检测意义、原理，观察中性粒细胞及其吞噬的葡萄球菌； （310室） 2、巨噬细胞的吞噬作用（大吞噬） 了解大吞噬试验检测意义、原理，观察巨噬细胞及其吞噬的鸡红细胞。 （310室） 吞噬百分率= ×100% 吞噬指数= ×100% 吞噬细菌的中性粒细胞数 观察记录的中性粒总数(100) 被吞噬的细菌总数 观察记录的吞噬细胞总数(100) Phagocytic cells monocyte neutrophil Phagocytic cells 中性粒细胞内的脑膜炎奈瑟菌_革兰染色 脑膜炎奈瑟菌 噬血细胞综合征 骨髓涂片中出现体积较大的噬血组织细胞，吞噬物