光盘-分光光度（- spectrophotometer of optical disk）.docVIP

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光盘-分光光度（- spectrophotometer of optical disk）.doc

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光盘-分光光度（- spectrophotometer of optical disk）.doc

光盘13-3分光光度（13-3 spectrophotometer of optical disk） The thirteenth chapter Spectrophotometry 1. write the formula of Lambert Bills law and explain what the symbols represent [answer] Lambert Beers law formula: A = kappa BC A is the absorbance; B is the thickness of the absorption cell (liquid layer thickness), the unit is cm; the C is the concentration of the substance measured (mol; L-1); the kappa is the molar absorption coefficient, the unit is L. Mol - 1. Cm-1. Lambert Beer law can also be expressed as: A = AB? Mass concentration (G? L-1); a is the mass absorption coefficient, and the unit is L. G – 1. Cm-1. A and kappa can be converted by the following: kappa = aM M in the formula represents the molar mass of the substance to be measured. 2., in order to improve the sensitivity and accuracy of spectrophotometry, what must be considered when designing experiments? [answer] in order to improve the sensitivity and accuracy of measurement, we must consider the following aspects: (1) the maximum absorption wavelength is chosen as the incident wavelength; (2) the ordinary spectrophotometer should measure the range of A = 0.2~0.7 by adjusting the concentration of the sample and the thickness of the liquid layer. 3. what is absorption spectrum? What is a standard curve? What are the practical applications of each method in spectrophotometry? [a] (1) the different wavelengths of monochromatic light is followed by the analysis of the material, the measured absorbance A under different wavelength to wavelength? As the abscissa and the A absorbance as ordinate, the availability of a curve is the absorption spectrum (absorption spectrum) (absorption curve) or absorption curve. Absorption spectrum reflects the characteristics of matter, and it is the basis of qualitative and quantitative analysis. The maximum absorption wavelength (max) can be determined in the absorption spectrum. In order to improve the detection sensitivity, Max wavelength is chosen as the incident wavelen