BAG-1CDK4蛋白在结直肠癌中表达及意义.doc

BAG-1CDK4蛋白在结直肠癌中表达及意义 　　[摘要] 目的:探讨BAG-1、CDK4蛋白在结直肠癌中的表达及其与结直肠癌临床病理特征的关系。方法:应用免疫组化SP法对80例结直肠癌组织、40例结直肠腺瘤组织及25例癌旁正常结直肠组织中的BAG-1、CDK4进行检测。结果:BAG-1、CDK4在结直肠癌中过度表达,阳性率分别为80.0%(64/80)和65.0%(52/80),并且BAG-1、CDK4与肿瘤的分化程度成反比。有淋巴结转移的结直肠癌BAG-1、CDK4的阳性率分别为96.7%(29/30)和83.3%(25/30),无淋巴结转移的结直肠癌阳性率分别为70.0%(35/50)和54.0%(27/50),两者比较,差异有高度统计学意义(P 　　[关键词] 结直肠癌;BAG-1蛋白;CDK4蛋白;免疫组织化学 　　[中图分类号] R735.3+7[文献标识码]A [文章编号]1673-7210(2010)03(a)-016-03 　　 　　Expression and significance of BAG-1 and CDK4 in the colorectal cancer 　　ZHANG Zhaoxia1, LIANG Jianfang2, ZHENG Huixia2, XIAO Hong2 　　(1.Division of Pathology, Fenyang College of Shanxi Medical University, Fenyang 032200, China; 2.Department of Pathology, the First Clinical Medical College of Shanxi Medical University, Taiyuan 030001, China) 　　[Abstract] Objective: To explore the expressions of BAG-1 and CDK4 protein in colorectal cancer and its relationship with clinicopathological features of colorectal cancer. Methods: BAG-1 and CDK4 protein were detected in 80 cases of colorectal cancer, 40 cases of colorectal adenomas and 25 cases of adjacent normal colorectal tissues by immunohistochemistry SP method. Results: The overexpression of BAG-1 and CDK4 in colorectal cancer were 80.0% (64/80) and 65.0% (52/80) respectively, BAG-1 and CDK4 were inversely proportional to the degree of tumor differentiation. The positive rates of BAG-1 and CDK4 were 96.7% (29/30) and 83.3% (25/30) in lymph node metastasis of colorectal cancer, and were 70.0% (35/50) and 54.0% (27/50) in no lymph node metastasis of colorectal cancer, they showed significant difference (P 　　1.2试剂 　　BAG-1、CDK4检测均采用SP法。兔抗人CDK-4单克隆抗体(原液)购自武汉博士德生物工程有限公司,兔抗人BAG-1单克隆抗体(原液)购自杭州华安生物技术有限公司,免疫组化染色SP试剂盒为Maxin公司产品,购自福州迈新生物公司。 　　1.3方法 　　所有标本常规脱水后,用10%中性甲醛溶液固定,石蜡包埋,连续切片,片厚4 μm,采用免疫组化SP法。BAG-1(1∶200)用热???复抗原(枸橼酸pH 6.0),CDK4(1∶50)采用微波处理修复抗原。石蜡常规脱蜡并水化,PBS缓冲液冲洗,过氧化酶阻断内源性过氧化酶的活性。每张切片加50 μl一抗,室温下孵育60 min,加50 μl生物素标记的二抗(SPKitC液),孵育10 min。加50 μl链亲和素-过氧化物酶溶液室温下孵育10 min,再加入新鲜配制的DA