孕鼠染铝对胎鼠脑皮质神经元影响.docVIP

孕鼠染铝对胎鼠脑皮质神经元影响 　　【摘要】 目的 探讨母体孕期染铝对胚胎脑神经发育的毒性作用。方法 4~5月龄SD大鼠，雌雄各30只，1:1合笼喂养，每天早晨查雌鼠阴栓，发现阴栓即进入实验（E0），将孕鼠随机平分为2组。染铝组：E0E18期间以AlCl3100 mg/（kg#8226;d）灌胃,正常对照组：以等量蒸馏水灌胃。取孕鼠18 d胚胎，每只孕鼠任取其胚胎2只，每小组子代30只。常规石蜡切片，经神经元特异烯醇酶（Neuron specific enolase,NSE）免疫组化染色。分别对胎鼠皮质Par1区内椎体细胞层NSE免疫组化染色阳性反应细胞进行计数，并用细胞形态学计量方法测量阳性反应产物的平均光密度。结果 染铝组胎鼠NSE免疫组化染色阳性细胞均明显少于对照组胎鼠(P 　　【关键词】 孕期；铝；神经元；子代 　　 　　The aluminium effect on neurons of embryo in aluminium exposure to pregnant rats 　　JIANG Dong，KANG Chaosheng.Department of Anatomy,Guiyang Medical University,Guiyang 550004,China 　　【Abstract】 Objective To investigate the developmental neurotoxicity of embryo in aluminum exposure to pregnant rats．Methods 30 male and 30 female SpragueDawley rats of 4~5 months age，the rats were mated，cheek up female rats,vaginal plug in the morning at every day，the day for discovering vaginal plug wss EO．Pregnant rats were randomly divided into two equal groups：the aluminum exposed group(pregnant rats were given A1C13 100 mg/(kg#8226;d) by digestive tract from EO to El8)，and normal control group(pregnant rats were given equal distilled water)．After traditional parafin section preparation，the embryonal brain were stained immunohistoneactively，and NSEpositive cells were quantitatively analyzed with cell morphometric tehnique．Results The numbers of NSE positive cells in the internal pyramidal layer of Par1 were obliously decreased in aluminum exposed group than that of normal control group，their average opiticaloptical density were obviously lower， respectively．Conclusion The numbers of Neurons are decreased in aluminum exposure to pregnant rats，whichmaybe lead to developmental neurotoxicity． 　　【Key words】 Pregnant rats；Aluminium ；Neurons；Embryo 　　 　　铝是环境中含量最丰富的元素之一。70年代以前，人们普遍认为铝不经胃肠道吸收，所以被广泛用于非处方药、混凝剂、食品添加剂及制作食具。??年来，铝的环境健康效应日益受到重视。有研究表明，某些神经系统疾病如早老性痴呆、帕金森病、阿尔茨海默病等与铝的毒作用有关［1］。铝是一种肯定的神经毒物，它可通过饮水、食品添加剂、药品等途径进入人体并在神经系统内蓄积，造成神经系统慢性退行性改变［2］。以往认为胚胎和胎儿对铝的生物利用度很低，较少关注铝对胚胎和胎儿的神经发育毒性作用。随着抗酸剂用以缓解孕期“消化不良”症状的普遍使用，而抗酸剂中铝的含量较高，人们