近红外光谱技术对创伤性脑水肿在体微创监测研究.docVIP

近红外光谱技术对创伤性脑水肿在体微创监测研究 　　[摘要] 目的 探讨利用近红外光谱技术对创伤性脑水肿的发生发展规律进行微创在体监测的新方法。方法 采用Feeney′s 自由落体撞击法建立大鼠急性局灶性脑挫裂伤模型，以近红外光谱技术和干湿相对密度法监测创伤后脑水肿的变化。结果 伤后1 h伤侧脑组织已发生水肿，伤后24～72 h伤侧脑水肿达高峰，随后逐渐减轻。对侧脑水肿的变化规律与伤侧相同，但水肿程度远轻于伤侧。近红外光谱监测的组织光学参数(μ′s)与脑水含量变化规律一致。结论 ?μ′s是监测创伤性脑水肿的良好指标，近红外光谱技术可以用于在体微创监测创伤性脑水肿的变化。 　　[关键词] 近红外光谱；创伤性脑水肿；微创监测；大鼠? 　　[中图分类号] R318；R-33［文献标识码］ A 　　［文章编号］ 1671-7562（2008）04-0221-04 　　 　　Study on mininvasive monitoring of traumatic brain edema ? 　　in vivo by 　　near-infrared spectroscopy?? 　　 HE Liang?1, YOU Yang?1, YANG Tian-ming?1, QIAN Zhi-yu?2, GUO Li-na?2, MAO Wen-lan?2,WU Qi?1 　　（1. Department of Neurosurgery, Zhongda Hospital, Southeast University,Nanjing210009, China ；? 　　2. Department of Biomedical Engineering, Nanjing University of Aeronautics and 　　Astronautics, Nanjing210016, China） 　　Abstract: Objective To explore a new method of mininvasive monitoring the development of traumatic brain edema in vivo. Methods Acute regional brain trauma was applied according to Feeney′s apparatus. The changes of brain edema were monitored by near-infrared spectroscopy (NIRS) technology and by measuring the brain water content. ResultsDistinct brain edema in injured areas was found at 1 hours after trauma, which reached a summit of severity at 24-72 hours after trauma, then gradually declining. Changes in the contralateral cerebral were the 　　same as injury areas, but far lighter. Both the organization optical parameters (μ′s), measured by NIRS, and the brain water content could monitor the development of brain edema. Conclusion μ′s is a good indicator for monitoring traumatic brain edema. NIRS could monitor the development of traumatic brain edema in vivo.? 　　Key words: near-infrared spectrometer ; traumatic brain edema; noninvasive monitoring；rats? 　　 　　(Meodern Medical Journal,2008,36:221-224) 　　 　　创伤???脑水肿继发于颅脑损伤，其主要变化为脑实质内液体成分增加，引起脑体积增大，是创伤后继发性脑损害的重要症状，如不能给予及时诊治，会引发一系列并发症,甚至导致脑疝形成、中枢性呼吸循环衰竭而死亡。? 　　动物实验中研究脑水肿的方法主要有干湿相对密度法、特殊相对密度法等［1-2］，但这些方法均不能用于临床实时监