基因克隆表达及功能研究1 ppt课件.pptVIP

Gene cloning, expression and functional study 基因克隆，表达及功能研究 vectors Cloning vectors: 克隆载体 to clone a gene in a vector Expression vectors: 表达载体 to express a gene from a vector Integration vectors: 整合载体 to integrate a gene in a genome through a vector Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids BACs 4 Eukaryotic vectors Cloning vectors: allowing the exogenous DNA to be inserted, stored, and manipulated mainly at DNA level. expression vectors: allowing the exogenous DNA to be inserted, stored, and expressed. Contains an origin of replication, allowing for replica