美国杰斐逊学马新亮教授在四医大的讲座报告之—医学论文写作技巧.ppt

Smart Writing The Structure of Biomedical Manuscript Title Short running title Abstract Keywords Introduction Materials and Methods Results Tables Figures Figure legend Discussion References TITLE ABSTRACT Keep in Mind So? These results demonstrated that in vivo administration of insulin activated Akt through the PI3-kinase-dependent mechanism and reduced post-ischemic myocardial apoptotic death. Phosphorylation of eNOS and the concurrent increase of NO production contribute significantly to insulin’s anti-apoptotic effect. How to Be Logical? Body of Manuscript Introduction-------------WHY? Methods------------------How? Results--------------------What? Discussion---------------So? INTRODUCTION Why are our topics important What are known What are unknown What are we going to (what we did and what we find Methods(How) RESULTS Discussion Discussion Discussion ???Copy??? Do Not Underestimate the Importance of the Title!!! Shortest Abstract for Your Entire Manuscript Why? How? What? So? There is a big difference between the abstracts For Manuscript and Conference Recent evidence from cultured endothelial cell studies suggests that phosphorylation of endothelial nitric oxide synthase (eNOS) via the PI3-kinase-Akt pathway increases NO production. The present study was designed to elucidate the signaling pathway involved in the anti-apoptotic effect of insulin in vivo and to test the hypothesis that phosphorylation of eNOS by insulin may participate in insulin’s cardioprotective effect following myocardial ischemia and reperfusion (MI/R). Why? Male Sprague-Dawley rats were subjected to 30 min of MI and 4 hr of R. Rats were randomized to receive vehicle, insulin, insulin + wortmannin or insulin + L-NAME. How? Treatment with insulin resulted in 2.6-fold and 4.3-fold increases in Akt and eNOS phosphorylation, and a significant increase in NO production in ischemic/reperfused myocardial tissue. Phosphorylation of Akt and eNOS and increase of NO production by insulin w