P物质作用下LS2脊髓星形胶质细胞活化参于慢性前列腺疼痛的调制.DOCVIP

完全氟氏佐剂诱导大鼠慢性前列腺炎疼痛模型中L5-S2脊髓中枢小胶质细胞的活化( 张恒1，沈文浩1，潘进洪1，王永权1，刘丽梅2，杨忠3，周占松1( （第三军医大学：1西南医院全军泌尿中心；2西南医院病理科；3神经生物学教研室，重庆400038） [摘要] 目的： 观察大鼠慢性前列腺炎疼痛模型中L5-S2脊段背角小胶质细胞活化的变化。方法： 通过前列腺完全弗氏佐剂（CFA）注射制作大鼠慢性前列腺炎疼痛模型，对照组注射生理盐水，观察时间为0、3、14天，用热痛阈测定法和前列腺病理进行疼痛模型鉴定，采用实时荧光定量RT-PCR检测L5-S2脊段后角中小胶质细胞标志物IBA－1的表达。结果：成功建立慢性前列腺炎疼痛大鼠模型，并观察到L5-S2 脊髓背角中存在小胶质细胞的活化。结论：慢性前列腺炎疼痛可以引起L5-S2脊髓中枢小胶质细胞活化，小胶质细胞异常活化可引起神经炎性疼痛，有可能与慢性前列腺炎疼痛的持续和泛化有密切关系。 关键词：前列腺痛；氟氏佐剂；小胶质细胞；脊髓中枢 Microglial cells activation of L5-S2 spinal cord in complete Freund’s adjuvant-induced prostatitis rats ZHANG Heng1, SHEN Wen-hao1, PAN Jin-hong1,WANG Yong-quan1, LIU Li-mei2, YANG Zhong3, ZHOU Zan-song1 (1 Department of Urology , Southwest Hospital, 2 department of pathology, Southwest Hospital, 3 Department of neurobiology, Third Military Medical University, Chongqing 400038 , China) Abstract : Objective To investigate the activation of microglial cells in L5-S2 spinal cord of rat chronic prostatitis pain model. Methods the rat chronic prostatitis pain model has been made by injecting CFA and the control made by injecting Sodium Chloride to prostate and demonstrated with tail flick threshold test and pathological changes of prostate , to observe activation of microglial cells . Results The rat chronic prostatitis pain model has been made by CFA injection successfully and microglial cells in L5-S2 spinal cord of model were irritated obviously. Conclusions In chronic prostatitis pain, the microglial cells, a immune cells in spinal cord, had been activated in L5-S2 spinal cord, and then probably resulted in the maintenance and enlargement of prostatitis pain. Key words : prostatitis pain; CFA;　 microglial cells; spinal cord 越来越多的研究证实胶质细胞与多种慢性疼痛的发生有密切关系，特别是脊髓小胶质细胞在病理性疼痛中通过分泌炎性介质因子而致痛[]，慢性前列腺炎疼痛是泌尿科常见的疾病，很多患者症状顽固，治疗困难，有资料表明表性慢性前列腺炎可能存在脊髓中枢神经调控的异常[]，但在慢性前列腺疼痛中是否存在小胶质细胞活化仍不清楚。本研究通过CFA注射制作大鼠慢性前列腺炎疼痛模型，观察L5-S2脊段是否存在小胶质细胞的活化。 1. 材料和方法 1.1 主要试剂和仪器 CFS购自美国Sigma公司。RT-PCR kit购自上海生工生物工程公司，引物合成由上海基康公司完成，SYBR green PCR core reagent kit 购自美