中欧预防控制口蹄疫2011年培训课件.ppt

Quality Assurance (QA) 质量保证 Internal Quality Control (IQC) 内部质量控制 IQC controls must standardised against Reference Controls 质控必需依据标准对照进行标准化 Charting IQC results (Graph Sheet) 质控结果以图表形式出示 Use of Low Positive controls important to confirm sensitivity 应用弱阳性对照来确认检测的敏感性 Important for test validation 质控对方法验证很重要 External Quality Assurance (IQA) 外部质量保证 Proficiency Testing (PT) 熟练程度测定 Samples from Reference Laboratory tested in your laboratory 在你们的实验室检测来自参考实验室的样品 Important for test validation IQA对于检验能力验证很重要 Suspect case of disease LOCAL CLINICAL OBSERVATION LABORATORY DIAGNOSIS (Local or NRL) New Developments in Diagnosis 诊断方法的新发展 Lateral-flow devices for FMDV antigen 检抗原的试纸条 Mobile PCR 移动PCR Isothermal assays 等温扩增检测技术 Faster (more rapid detection) 更快速 Ability to support diagnosis based on clinical signs 支持临床诊断 疑似病例 临床观察 实验室诊断 Lateral-flow devices for FMDV Antigen detection 检测FMDV抗原的试纸条 Developed by IAH in collaboration with Brescia and Svanova 由IAH,Brescia与Svanova合作研发 Quick and simple to perform 快速简单 Pan-serotypic 适用于多个血清型 During 2007: used for rapid (10 mins) confirmation of FMD in the field 2007年：用于快速确认田间疫情（小于10min） Also useful in the Lab 也可用于实验室 LFD marketed by 由Svanova销售 Progress with the Enigma Diagnostics FL 实时荧光检测技术 Enigma have licence to use TaqMan? PCR technology basis for routine assays used in most diagnostic labs (FMDV, ASFV, BTV, BVDV, CSFV etc…) 2009-2010: Evaluation of machine’s capability at IAH wet assay with MagNA PURE reagents performance can be equivalent to routine rRT-PCR assays used in the WRL Results in 60 minutes （60min内出结果) Future project to develop a dry-down consumable for FMDV? Nucleic acid amplification at a single temperature 等温条件下扩增 No need for fragile precision instrumentation 不需精密设备 More suitable for use in the field 适合野外条件 Very rapid, similar sensitivity to rRT-PCR 快速，与实时荧光PCR敏感性相似 Isothermal amplification (RT-LAMP) 等温扩增技术 Dukes et al., 2006 James et al., 2010 A