青海东南部地区山丹百合遗传多样性研究-园林植物与观赏园艺专业论文.pdf.docxVIP

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青海东南部地区山丹百合遗传多样性研究-园林植物与观赏园艺专业论文.pdf.docx

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青海东南部地区山丹百合遗传多样性研究-园林植物与观赏园艺专业论文.pdf

AbstractL.pumilumDC.isperennialbulbflowerbelongedtoLiliuminLiliaceae.28populationswithinL.pumilumDC.werestudiedwithecologicalmarker,morphologicalmarker,biochemicalmarkerandmolecularbiologicalmarkeraccordingtotheprinciplesofpopulationbiology.Thegeneticdiversitywerediscussed.Themainresultswereasfollows:Greatdiversitieswereshowedbetween28populationsofL.pumilumDC..Therewerethreemainlymigratoryhighway,Astimewenton,respectivepopulationswereappeared.Analysisforallphenotypictraitsshowedthattherewassignificantdifferentwithinandamongpopulations.Greatdiversitieswereshowedbetween28populationsofL.pumilumDC..Themainphenotypicdifferentiationcoefficientforthe11traitsis79.66%,andthevariationamongpopulationswashigherthanwithinpopulation,whichindicatedthatthevatianceamongpopulationswasthechiefelementofphenotypicvariationofL.pumilumDC.insoutheastareaofQinghaiprovince.Themorphologicalvariationclusteringannalysisindicatedthat28populationsweredividedintofourbiggroups.Comparedwithecologyclustering,resultsofthistwomarkerswereanalogical,morethan80%wasthesame.Itshowedthatvariationofphenotypictraitswassequential.Ittallyedwithfieldnote.ForPODdiversityananlysis,DicesimilaritybetweenpopulationsofL.pumilumbasedonPODmarkerwere0.333to1.000,averagewas0.6615.GreatdiversitieswereshowedbetweenpopulationsofL.pumilum.ZLwasthehighestvariancepopulationwhileYSwasthelowest.The12populationsweredividedinto3biggroupsandarespectivepopulation.Comparedwithmorphologicalmarker,75%ofthemwerethesame.Therewere147polymorphiclociamong152ISSRlociof15primesselectedfrom123ISSRprimers.Thetotalpercentageofpolymorphicloci(PPL)was96.71%.TherangeofPPLof28populationswerefrom50%to74.34%.Itindicatedthatthereweregreatdivesitiesbetweenpopulations.AsanalyzedbyPOPGENE,thegeneticdifferentiationcoefficient(Gst)amongpopulationswas0.6915．Andthegeneticdifferentiationcoefficientamongpopulations((Hsp-Hpop)/Hsp)calculatedbytheShannondiversityindexwas0.6336.Thesedatademonstratedthatthegeneticvariationofbetween-populationaccountsforthemostofgenet