珠子参多糖抗肝癌作用的实验.docVIP

使用前删除此我爱浪漫樱花文档主页： HYPERLINK /mtl168168 /mtl168168，分享文档快乐生活快乐无阻PAGE PAGE 1浅谈????????????????????? 作者：陈涛，陈茂华，胡月琴，巩仔鹏【摘要】? 目的观察珠子参多糖(panax japlcus var polysaccharide,pjps)对小鼠h22肝癌移植瘤生长的抑制效应及其机制。方法分别建立balb/c小鼠h22肝癌移植实体瘤模型及腹水瘤模型，将小鼠随机分为3组，即生理盐水组(阴性对照）、5-氟尿嘧啶组（阳性对照）、珠子参多糖组。给药结束后比较各用药组的抑瘤率、胸腺指数、脾指数、肿瘤组织细胞形态结构，肿瘤细胞超微结构、肿瘤细胞凋亡率、cd4+/cd8+值、外周血血清中血管内皮生长因子含量及生命延长率。结果珠子参多糖组的胸腺指数、脾指数、cd4+/cd8+值，外周血血清中血管内皮生长因子含量及生命延长率均高于5-氟尿嘧啶组，珠子参多糖组的抑瘤率及肿瘤细胞凋亡率低于5-氟尿嘧啶组，珠子参多糖组的部分肿瘤细胞有坏死现象及凋亡学形态变化。结论珠子参多糖对h22肝癌小鼠具有抑制肿瘤生长、延长生命的作用。提高机体细胞免疫功能，干扰肿瘤细胞周期，降低vegf的表达可能是珠子参多糖发挥抗肝癌效应的机制。 【关键词】? 珠子参； 多糖； 肝癌； 肿瘤抑制　　abstract：objectiveto observe the anti-tumor activity of panax japlcus var polysaccharide (pjps) on transplanted tumor h22 in mice and its mechanism.methodsh22 transplanted solid hepatocarcinoma model and the ascites hepatocarcinoma model were established.the balb/c mice were randomly divided into 3 groups:normal saline group(negative control group)，5-fluorouracil group(positive control group),and pjps group. after administration,the inhibitory rate of tumor,the thymus index ,the spleen index,tumor cell morphology,and ultrastructure,the rate of apoptosis of tumor cell,cd4+/cd8+ ratio,level of vegf and the rate of life extension were determined. resultsthe thymus index,spleen index,cd4+/cd8+ ratio,level of vegf and the rate of life extension in pjps group were higher than those of 5-fu group,but the inhibiting rate of? tumor and the apoptosis rate of tumor cell were lower than those of 5-fu group.the tumor cells of pjps group partly died . the tumor cell nucleus of pjps group concentrated, and tumor cells got smaller. conclusionpjps can inhibit the growth of hepatoma carcinoma cells of h22 tumor-bearing mice. improving the cellular immune function, interfering with tumor cell cycle and reducing the expression of vegf may be the mechamisms of antitumor effect. 　　key words： panax japlcus var ;?? polysaccharide ;? liver cancer;? tumor suppressor ??? 珠子参panax japlcus var,major又名扣子七，属五加科人参属植物，主要分布于东喜马拉雅