概述发展历史研究方法英文版.PPT

Introduction 1.???Goal of Histology Histology is the study of the tissue of the body and of how these tissues are arranged to constitute organs. Cell: Tissue: made of cells and extracellular matrix Basic tissue: Epithelium Connective tissue Muscle tissue Nerve tissue cells and extracellular matrix Tissue: Organ: systems : 2. Relationship between histology and other medicine courses 3. Histologic methods 3-1. microscopy/显微镜 Fine structure light microscopy: (LM) resolution—0.2um 1um＝10－3mm＝10－6m H.E. Staining ( H: Hematoxylin E : Eosin ) basophilic, acidophilic Electronmicroscopy (EM) : ultra structure resolution---0.2nm / 1um=1000nm scanning EM transmission EM Histochemistry and Cytochemistry 过碘酸－Schiff反应：（periodic acid Schiff reaction，PAS 反应） 过碘酸 糖－乙二醇基  Immunohistochemistry, Immunocytochemistry 3-3 Cell and Tissue Culture 组织工程（tissue engineering） 我国第一个组织工程产品——组织工程皮肤 3-4 Hybridization Techniques In situ Hybridization 原位杂交 4. Problems in the interpretation of tissue section * * 孔 力 Human Histology Histology and Embryology Department Epithelium Connective tissue Muscle tissue Nerve tissue 激光共聚焦扫描显微镜 （confocal laser scanning microscope） 激光共聚焦扫描图像 Photomicrograph of an intestinal villus stained by PAS. Staining is intense in the cell surface brush border (arrows) and in the secretory product of goblet cells (G) because of their high content of polysaccharides. The counterstain was hematoxylin. 3-2 Specific Localisation Methods for LM and EM 糖－乙二醛基 Schiff试剂 （亚硫酸品红） 不溶性紫红色反应产物 （ PAS 反应