人-NOD／SCID小鼠异种急性移植物抗宿主病模型-中国动物学报.DOCVIP

人脐带间充质干细胞静脉输注小鼠的安全性何君1,2，李洋1,2，陈威1,2，郝好杰3，李名烁1,2，韩瑞红1,2，武鑫1,2，卢星辰1,2，金翠英1,2，郭珣1,2，易辉1,2，李晓岑1,2，赵颖1,2，武岩1,2，徐玉环1,2 1.中国医学科学院医学实验动物研究所，卫生部人类疾病比较医学重点实验室，北京，1000212.中国医学科学院糖尿病研究中心，北京，1000053.解放军总医院生命科学院基础医学所，北京，100853【摘要】目的 探讨C57/BL6J小鼠重复多次尾静脉输注人脐带间充质干细胞后的免疫反应和毒性。方法 将SPF级别的32只C57/BL6J小鼠随机分为阴性对照组、细胞移植组，每组16 只，雌雄各半，细胞移植组小鼠尾静脉注射分离培养的第5代人脐带间充质干细胞，一次5×106/只，每周注射一次，连续注射4周；阴性对照组每次注射相同容积的PBS。注射后后观察小鼠的一般症状，末次注射后1周、4周进行血细胞计数、血生化、免疫反应指标、脏器质量测定和组织病理学检查。结果 细胞移植组小鼠血细胞计数、血生化、脏器重量和脏器系数与对照组无显著性差异（P 0.05），脏器组织病理学在光镜下检查结果与对照组无形态学差别，以及免疫结果测定（T细胞亚群CD3+、CD4+、CD8+及CD4+/CD8+）与对照组无显著性差异( P 0.05)，较对照组有显著性差异（P?0.01）。 结论 人脐带间充质干细胞重复多次尾静脉输注C57/BL6J小鼠是安全可行的，对受者无明显免疫反应和毒副作用。【关键词】人脐带间充质干细胞；静脉输注；重复多次【中图分类号】 R332 A safety study of intravenous injection of human umbilical cord mesenchymal stem cells in miceHE Jun1,2, LI Yang1,2, CHEN Wei1,2, HAO Hao-jie3, LI Ming-shuo1,2, HAN Rui-hong1,2, WU Xin1,2, LU Xing-chen1,2, JIN Cui-ying1,2, GUO Xun1,2, YI Hui1,2, LI Xiao-cen1,2, ZHAO Ying1,2, WU Yan1,2, XU Yu-huan1,2, 1.Institute of Laboratory Animal Science，Chinese Academy of Medical Science，Beijing 100021，China2.Diabetes research center，Chinese Academy of Medical Science，Beijing 100005，China3.Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital，Beijing 100853，China[Abstract] Objective: To study the toxicity of intravenous injection of human umbilical cord mesenchymal stem cells in C57/BL6J mice with repeated administrations. Methods: 32 SPF C57/BL6J mice were randomly divided into negative control group and HUMSC transplantation group, of equal gender. HUMSCs were isolated and subsequently cultured through 5 passages in vitro. 2×106 HUMSCs were injected each mice of transplantation group via tail vein once 1 week for 4 weeks, and the equal quantity PBS were injected to control group mice. Mice were euthanatized on the day of 1 week (8 mice per group) and 4 weeks(all survived mice) after the last treatment following the schedule. Toxicity was evaluated by clinical observ