各类藻类培养基详解.docVIP

AJS (Acidified JM:SE)Freshwater alga Dunaliella acidophilaMedium Acidified 97:3 mixture of JM and SE2See separate recipes( HYPERLINK http://www.ife.ac.uk/ccap/MediaRecipes2.html \l JM JM and HYPERLINK http://www.ife.ac.uk/ccap/MediaRecipes2.html \l SE2 SE2). For approximately 1?litre of final medium, mix 970?ml JM with 30?ml SE2. Add 10?ml of conc H2SO4 to give a pH of approximately 1.5. Autoclave at 15 psi for 15 minutes.ANT (Antias Medium)Marine algae Stock Trace metals stock solution (chelated) per litre EDTANa2.2H2O 3.24 g FeCl3.4H2O 1.08 g MnSO4.4H2O 0.450 g ZnSO4.7H2O 0.230 g Na2MoO4.2H2O 0.097 g CuSO4.5H2O 0.01 g CoSO4.7H2O 0.0056 gMake up to 1 litre with distilled water and adjust pH to 7.6 - 7.8 with dilute HCl or NaOH. Store frozen. Medium per litre KNO3 0.05 g NaH2PO4.2H2O 0.0078 g Tris [tris(hydroxymethyl)aminomethane] 1.0 g Glycine 0.3 g Trace metals stock solution (chelated) 2.5 ml Thiamine HCl 500.0 μg Cyanocobalamin 2.0 μg Biotin 1.0 μg Filtered natural seawater 800.0 mlMake up to 1 litre with distilled water and autoclave at 15 psi. Final pH should be 7.6 - 7.8. Ref HYPERLINK http://www.ife.ac.uk/ccap/References.html Antia, Cheng Taylor (1969) ASW (