油菜种子中胰蛋白酶抑剂之纯化.docVIP

PAGE PAGE 19參考範本文章圖、表請隨文插入，勿分置二頁靠右對齊Times New Roman10最小行高18pt靠左對齊標楷體10參考範本文章圖、表請隨文插入，勿分置二頁靠右對齊Times New Roman10最小行高18pt靠左對齊標楷體10最小行高18ptJournal of YuanpeiScience and TechnologyNo.10, December 2003P.13 ～ P.22元 培 學 報第十期 民國92年12月13 ～ 22 頁置中標楷體24單行間距置中標楷體24單行間距Times New Roman16置中Times New Roman18固定行高23Purification and Characterization of a Trypsin Inhibitor Times New Roman16置中Times New Roman18固定行高23洪志宏＊Corresponding author Chih-Hung Hung＊Corresponding authorE-mail: HYPERLINK mailto:chihhung@.tw chihhung@.tw黃兆君 Chou-Chun HuangE-mail: chouchun@.tw蔡文翔 Wein-Shiang TsaiE-mail: twspal@.tw王海龍 Hai-Lung Wang標楷體16固定行高16ptE-mail: HYPERLINK mailto:Hailung@.tw Hailung@.tw標楷體16固定行高16pt陳媛孃 Yuan-Liang ChenE-mail: HYPERLINK mailto:yunliang@.tw yunliang@.tw元培科學技術學院醫事技術系Times New Roman10Department of Medical Technology, Yuanpei Times New Roman10(Received October 29, 2003; Revised December 3, 200新細明體10粗體邊界上2.5㎝下3㎝左右2.4㎝左右對齊新細明體10固定行高16pt摘 要：許多研究報告，癌細胞之產生、侵襲、轉移與蛋白酵素抑制劑有很大的關係，當外加蛋白酵素抑制劑或誘發內生性隻蛋白酵素抑制劑可以改善這些現象，故尋找及利用蛋白酵素抑制劑是極具研究意義。在本研究中，我們由油菜(Brassica campestris)種子純化出一種胰蛋白酶抑制劑（Brassica campestris trypsin inhibitor, BCTI），並做了一般性質之探討，純化的過程是利用硫酸銨分割，經透析及活性分析，得知其活性介於硫酸銨分割20-50﹪之間，接著將樣品通過DE-52 cellulose陰離子交換樹脂及trypsin-Sepharose 4B親和性管柱，所純化出之BCTI以SDS分析，得知其分子量約8 kDa，是屬於Bowman-Birk 型蛋白酶抑制劑。進一步對此蛋白的性質研究，發現此蛋白對熱及變性劑(SDS)具有非常高的耐受性，90℃新細明體10粗體邊界上2.5㎝下3㎝左右2.4㎝左右對齊新細明體10固定行高16pt關鍵字：Bowman-Birk 型蛋白酶抑制劑、胰蛋白酶抑制劑、油菜英文內文左右對齊Times New Roman10 固定行高16pt縮寫：BCTI，Brassica campestris trypsin inhibitor；BAPNA，(N-benzoyl-DL-arginyl-英文內文左右對齊Times New Roman10 固定行高16ptAbstract： A trypsin inhibitor (BCTI) was purified from seeds of Brassica campestris by 20-50% ammonium sulfate precipitation, DE-52 ion-exchange column and trypsin-Sepharose 4B affinity chromatography. A molecular weight of 8 kDa was estimated by SDS. The BCTI was found to be a thermostable Bowman-Birk type TI that inhibits trypsin at molar ratio 1:1. The stability of BCTI was studied by exposing it to altered conditions of temperature and protein-