化脓菌肠道菌分鉴定过程（四）.pptVIP

甘露醇发酵试验 药敏实验 Bacteriological Detection Serological diagnosis Samples: Widal’s test ------ S. typhi and paratyphi Weil-Felix Test ------ Richettsia(立克次体属) Widal’s Reaction Definition: a specific reaction consisting in agglutination of typhoid bacilli or paratyphoid bacilli when mixed with serum from a patient having typhoid fever or other salmonella infection and constituting a test for the disease . Usage: to assist to diagnosis typhoid fever or other salmonella infection. Materials Antigens: TO TH PA PB Antibody: serum of suspected patient Normal saline Reaction plate Straws PROCEDURE Make the mark Add reagents as the following: Reagent (ml) 1 2 3 4 5 6 7 Observation: 1. Control tube: no agglutination (-) 2. Lowest titer tube: absolutely agglutination (++++) 3. Other tubes: three quarters agglutination(+++) half agglutination (++) one quarter agglutination (+) no agglutination (-) Judgment: Agglutination titer: the highest dilution of serum which appears (++) bacteria agglutination. Precautions Make the mark Make sure the procedure carefully use the straws Correctly Judge the results 细菌特殊结构观察 荚膜 荚膜染色法：菌体紫色，荚膜无色或淡紫色 芽孢 芽孢染色法：菌体蓝色，芽孢红色 鞭毛 鞭毛染色法：菌体红色，鞭毛红色 异染颗粒 异染颗粒染色法：菌体浅黄色，异染颗粒深蓝紫色 抗酸染色 抗酸性细菌：红色 非抗酸性细菌：蓝色 模拟题 在SS琼脂平板上，致病性肠道杆菌菌落特征是____________，非致病性肠道杆菌的菌落特征是____________。 斜面培养基主要用于 __________；半固体培养基主要用于__________。分离肠道杆菌常用_________培养基。 现有一名患者，伤口化脓性感染，请问你如何进行鉴定？ * * 化脓菌/肠道菌分离鉴定过程（四） 细菌的抑菌环直径解释标准和相应的MIC解释标准（来源于CLSI M100-S17和NCCLS M31-A2） ≥18 15～17 ≤14 30 1,2 头孢他啶?Ceftazidime ≥21 14～20 ≤13 30 1,2 头孢曲松?Ceftriaxone ≥21 16～20 ≤15 75 1,2 头孢哌酮?Cefoperazone ≥18 15～17 ≤14 30 1,2 头孢噻吩?Cephalothin ≥18 15～17 ≤14 30 1,2 头孢唑啉?Cefazolin 头孢类 ≥18 - ≤17 100/10 2 ≥21 18～20 ≤17 100/10 1 哌拉西林/他唑巴坦?Piperacillin/Tazobactam ≥15 12～14 ≤11 10/10 1,2 氨苄西林/舒巴坦?Amplicillin/Sulbactam ≥20 - ≤19 20/10 2 ≥18 14～17 ≤13 20/10 1 阿莫西林/克拉维酸?Am