ELISA法和化学发光法在血清HIV―1HIV―2HCV抗体检测中应用价值分析.docVIP

ELISA法和化学发光法在血清HIV―1HIV―2HCV抗体检测中应用价值分析 　　[摘要]目的 分析酶联免疫吸附测定法（ELISA）和化学发光法（CLIA）对血清中HIV-1抗体、HIV-2抗体、HCV抗体的检测意义。方法 选取2016年3月～2017年6月我院收集的血液标本1188份，分别采用ELISA法和CLIA法对标本进行HIV-1抗体、HIV-2抗体、HCV抗体进行检测，以Western Blot检测结果作为HIV-1抗体、HIV-2抗体检测金标准，以PCR检测结果作为HCV抗体检测金标准。比较两种方法的诊断效果。结果 在检测HIV-1抗体、HIV-2抗体时，CLIA法与金标准Western blot的Kappa值为0.923，高于ELISA法与金标准Western blot的0.768。CLIA法的Youden指数为0.999，高于ELISA的0.832。在检测HCV抗体时，CLIA法与金标准PCR的Kappa值为0.898，高于ELISA法与金标准PCR的0.831。CLIA法的Youden指数为0.905，高于ELISA的0.831。结论 CLIA法检测血清HIV-1、HIV-2、HCV时具有灵敏度、特异度较高，与金标准高度一致的特点，值得临床推广。 　　[关键词]酶联免疫吸附测定法；化学发光法；人类免疫缺陷病毒；丙型肝炎病毒 　　[中图分类号] R512.91 [文献标识码] A [文章编号] 1674-4721（2018）1（c）-0135-04 　　Application value analysis of ELISA and Chemiluminescence in detection of serum HIV-1，HIV-2 and HCV antibodies 　　HUANG Yan-qing LIN Yu-ye 　　Clinical Laboratory，People′s Hospital of Lancheng District of Jieyang City in Guangdong Province，Jieyang 522000，China 　　[Abstract]Objective To analyse the significance of ELISA and CLIA value on serum HIV-1 antibody，HIV-2 antibody，HCV antibody detection.Methods 1188 blood samples collected in our hospital from March 2016 to June 2017 were selected.HIV-1 antibody，HIV-2 antibody and HCV antibody were detected by ELISA and CLIA respectively.Western blot test results as HIV-1 antibody，HIV-2 antibody test gold standard，PCR test results as HCV antibody test gold standard，the diagnostic effects of both methods were compared.Results In the detection of HIV-1 antibody，HIV-2 antibody，the Kappa of CLIA method with Western blot was 0.923，which was higher than that of the ELISA method （0.768）.The Youden index of CLIA was 0.999，which was higher than that of the ELISA （0.832）.In the detection of HCV antibody，the Kappa of CLIA method with Western blot was 0.898，which was higher than that of the ELISA method （0.831）.The Youden index of CLIA was 0.905，which was higher than that of the ELISA （0.831）.Conclusion In the detection of HIV-1，HIV-2，HCV，the sensitivity，specificity of CLIA is higher，and more consis