慢性丙肝及肝硬化患者外周血Th17Treg细胞及其相关细胞因子表达及意义.docVIP

慢性丙肝及肝硬化患者外周血Th17Treg细胞及其相关细胞因子表达及意义 　　[摘要] 目的 研究慢性丙肝及肝硬化患者外周血Th17细胞、Treg细胞及其相关因子的表达及意义，分析Th17细胞、Treg细胞及其相关因子的变化对患者的影响。 方法 选取2014年3月～2016年2月在运城市中心医院进行治疗的慢性丙肝患者47例，肝硬化患者27例，同时从健康人群中选取39人，分成慢性丙肝组、肝硬化组和健康对照组。用流式细胞检测三组患者Th17细胞和Treg细胞表达率，用ELSIA技术检测IL-10、 IL-17、IL-6、TGF-β的血清水平，比较三组之间的差异。 结果 健康对照组Th17细胞表达率及IL-6、IL-17水平明显低于慢性丙肝组，健康对照组Treg细胞表达率及IL-6水平明显低于慢性丙肝组和肝硬化组，健康对照组和慢性丙肝组Th17/Treg比值明显高于肝硬化组，健康对照组和慢性丙肝组TGF-β、IL-10水平明显低于肝硬化组，差异均有统计学意义（P0.05）。 结论 Th17细胞和Treg细胞表达率以及相关因子的血清水平的变化与慢性丙肝及肝硬化有密切的关系，关注Th17细胞和Treg细胞表达率以及相关因子的血清水平的变化对预防慢性丙肝及肝硬化有重要指导意义。 　　[关键词] 慢性丙肝；肝硬化；Th17细胞；Treg细胞；细胞因子 　　[中图分类号] R512.6 [文献标识码] B [文章编号] 2095-0616（2017）15-248-04 　　[Abstract] Objective To study the expression and significance of Th17 cells， Treg cells and related factors in peripheral blood of patients with chronic hepatitis C and cirrhosis， and to analyze the effect of Th17 cells， Treg cells and their related factors on the patients. Methods 47 patients with chronic hepatitis C and 27 patients with cirrhosis treated in Yuncheng Central Hospital from March 2014 to February 2016， and 39 healthy subjects were selected. They were divided into chronic hepatitis C group， cirrhosis group and healthy control group. The expression rates of Th17 cells and Treg cells were detected by flow cytometry. Serum levels of IL-10， IL-17， IL-6 and TGF-β were measured by ELSIA technique. The differences between the three groups were compared. Results The expression of IL-6 and IL-17 in healthy control group was significantly lower than that in chronic hepatitis C group. The expression rate of Treg cells and IL-6 level in healthy control group were significantly lower than those in chronic hepatitis C group and cirrhosis group. The ratio of Th17/ Treg in chronic hepatitis C group was significantly higher than that in cirrhosis group， and the level of TGF-β and IL-10 in healthy control group and chronic hepatitis C group was significantly lower than that in cirrhosis group （P0.05）. Conclusion The expression of Th17 ce