叶黄素玉米黄素分子结构修饰对抑制肿瘤细胞增殖活性的影响.PDF

296 2007, Vol. 28, No. 04 食品科学 ※营养卫生 孙 震，姚惠源* (江南大学食品学院，食品科学与安全教育部重点实验室，江苏 无锡 214036) ：目的：探讨叶黄素、玉米黄素的结构与抗肿瘤效果之间的关系。方法：以从玉米蛋白粉中分离、提 取的叶黄素和玉米黄素为原料，通过化学合成的方法分别对其所携带的紫罗酮环上的羟基进行单、双乙酰化修饰， 所合成的产物用紫外可见光扫描，液质联用，核磁共振等手段分子结构的鉴定；用MTT(噻唑蓝)的方法测定各叶 黄素、玉米黄素结构被修饰前后抑制人口腔上皮细胞癌KB 增殖活性的变化。结果：叶黄素、玉米黄素分子紫罗 酮环上的羟基被乙酰基取代后，其抑制细胞增殖的活性有所降低。结论：叶黄素和玉米黄素分子结构中羟基取代 基的存在对其调节肿瘤细胞的增殖能力有一定的贡献。 ：叶黄素；玉米黄素；结构修饰；叶黄素双乙酰化酯；玉米黄素双乙酰化酯；抗肿瘤活性 Effects of Molecule Structure Modification of Lutein and Zeaxanthin on Inhibitory Tumor Cell Proliferation Activity SUN Zhen，YAO Hui-yuan* (Key Laboratory of Food Science and Safety, Ministry of Education, Southern Yangtze University, Wuxi 214036, China) Abstract ：This study was aimed at investigating the relationship between lutein and zeaxanthin structures and their anti-tumor effects. Methods: Zeaxanthin and lutein were purified from corn protein residues, and then structures modified by mono- and di- acetylation of their ionone ring hydroxyl groups. The structures of the modified products were characterized by means of UV-visible absorption spectroscopy, HPLC-mass spectroscopy and 1H-NMR spectroscopy. The tumor inhibitory effects of mono- and di-acetylated zeaxanthin and lutein were compared with that of natural zeaxanthin and lutein by MTT(methylthiaz olydiphenyl-tetrazolium bromide) assay with human mouth epithelial cancer KB line as model cells. Results: Zeaxanthin and lutein showed lower anti-tumor-proliferation activities after mono- and di- acetylation of their ionone ring hydroxyl groups. Conclusion: The hydroxyl groups in zeaxanthin and lutein molecules make significant contribution to the function of anti-tumor-proliferation