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网址： HYPERLINK 第 PAGE 1 页，共 NUMPAGES 1 页 沙眼衣原体的基因分型及其临床意义 沙眼衣原体的基因分型及其临床意义 中华皮肤科杂志 1998年第3期第31卷 论著 作者：王千秋　叶顺章　钟铭英　张树文 单位：210042 南京，中国医学科学院 　中国协和医科大学皮肤病研究所［王千秋　叶顺章(指导者)　钟铭英　张树文］ 关键词：沙眼衣原体；聚合酶链反应；基因型 【摘要】目的　探讨对沙眼衣原体更为简便的分型方法及基因分型的临床意义。方法　利用聚合酶链反应(PCR)扩增沙眼衣原体外膜蛋白基因(omp1)片段，以核酸内切酶作酶切，10%聚丙烯酰胺凝胶电泳，银染色后观察带型分布。结果　扩增15种血清型omp1基因，产物为871bp 的DNA片段。用AluⅠ酶切此片段，观察到15种血清型呈现具有特征性的带型分布，但C组带 型 较难区别。再用HpaⅡ、HinfⅠ及EcoRⅠ三重酶切，则可将C组各型完全区分开来。对74株沙眼衣原体临床株进行分型，发现E,F,G,D在本观察人群中占优势，偶尔，也见到B,H,J等型别。还观察到1例F/D混合型。沙眼衣原体的型别与临床表现之间未见有明显的关联，但发现D型 感染者常为抗沙眼衣原体抗体高滴度者。结论　omp1基因分型技术可以作为流行病学调查中的有用的研究工具。 Genotyping of Chlamydia trachomatis Isolates and Its Clinical Si gnificanceWang Qianqiu, Ye Shunzhang, Zhong Mingying, et al. Instit ute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medic al College, Nanjing 210042 Abstract　Objectives　To test a simple method for genotyping of C.trachomatis isolates and to investigate the clinical signi ficance of the genotypes. Methods A part of the chlamydial genome enco ding the majo r outer membrane protein(omp1) was amplified by polymerase chain reaction (P CR). The products were digested by endonucleases to see the characteristic pa tterns, after silver staining on 10% polyacrylamide gels.Result s　 The omp1 genes of 15 serovars of C. trachomatis were am pl ified by PCR,which generated an 871 base pair gene fragment. AluⅠ digestion of the product gave characteristic patterns for the 15 serovars,but group C presented closely similar patterns. A triple digestion with HpaⅡ, foll owed b y HinfⅠ and EcoRⅠ, would allow the differentiation of serovars in group C. Ana lysis of 74 clinical isolates revealed serovars E, F, D, G as the most prevalen t gen ital serovars in the studied populations. Serovars B, H, J were occasionally id entified. A mixed infection with serovars F and D was seen in a clinical samp le. No significant relationship was observed between clinical manifestations of ur