房山封闭群小型猪微卫星位点的测定.DOCVIP

房山封闭群小型猪微卫星位点的测定 魏杰1，王吉星*2，王洪1，李芳芳1，岳秉飞1 （1 中国食品药品检定研究院，北京 100050；2 北京实验动物行业协会，北京 100195） [摘要] 目的 依据北京市地方标准DB11/T828.3-2011，在DNA水平上对房山封闭群小型猪进行种群遗传质量评价与分析。 方法 按照北京市地方标准DB11/T828.3-2011规定，利用25对微卫星引物，对22头房山封闭群小型猪进行PCR检测，利用群体遗传分析软件Popgen32对所得结果进行处理。 结果25对引物中，4对引物经反复调整PCR反应条件不能得到稳定扩增结果，其余21对引物得以稳定扩增；共得到91个有效等位基因型，平均多态性信息含量（PIC）为0.4630，平均杂合度为0.5331。同时，SWr1008位点上的产物与DB11/T828.3-2011标准有所差异；标准对SW271位点所在染色体报道也与美国农业部官网等数据库不同。 结论 房山封闭群小型猪群体具有一定的遗传稳定性，符合封闭群动物的遗传结构特征。 [关键词] 微卫星，小型猪，封闭群，多态性，遗传检测，地方标准，适用性 [中图分类书号] R -33 [文献标识码]A [文章编号] Study on DNA polymorphisms of microsatellite locus in Fangshan closed colony miniature pigs WEI Jie1, WANG Ji-xing*2, Wang Hong1, LI Fang-fang1, YUE Bing-fei1 ( 1.National Institutes for Food and Drug Control,Beijing 100050, China;2. Beijing Laboratory Animal Industry Association, Beijing 100195, China) [Abstract] Objective To analyse and evaluate the genetic quality of Fangshan closed colony miniature pig population in DNA level according to DB11/T828.3-2011. Methods According to the local standard DB11/T828.3-2011, 25 pairs of microsatellite primers were used in 22 Fangshan closed colony miniature pigs for HYPERLINK app:ds:polymerasepolymerase HYPERLINK app:ds:chainchain HYPERLINK app:ds:reactionreaction. Popgen32 was used for result handler. Results Among 25 pairs of primers, 4 of that were not able to get stable amplification results, even when changing HYPERLINK app:ds:reaction \t reaction HYPERLINK app:ds:condition \t condition; 21 pairs of primers were able to get stable amplification results. There are 91 valid allelotype in 21 microsatellite locus. The average PIC is 0.4630, and the average HYPERLINK app:ds:heterozygosityheterozygosity is 0.5331. What’s more, products of SWr1008 is different from that of DB/T828.3-2011. And the chromosome position of SW271 in DB/T828.3-2011 and HYPERLINK also HYPERLINK app:ds:exist \t exists HYPERLINK app:ds:differences \t differences. Conclusion Fangshan closed colony miniature