产妇子宫平滑肌大电导钙激活钾通道(BKCa)表达水平变化及其与小窝蛋白相互作用和产后出血发病关系-妇产科学(围产医学)专业论文.docxVIP

PAGE PAGE 6 myometrium were significantly higher than in control group(P0.05). ③ Both groups, BKCa (α, β subunit) protein and mRNA level in myometrium was negatively related to the level of myometrium contraction. ④ Both groups, BKCa(α subunit), caveolin-1 and caveolin-2 were detected in myometrium; BKCa(α subunit) localized primarily on the plasma membrane, whereas caveolin-1 and caveolin-2 localized to intracellular and pasma membrane compartments; BKCa(α subunit) and caveolin-1, BKCa(α subunit) and caveolin-2 co-immunolocalized on part of the myometrial smooth muscle cell membrane. ⑤ In study group, both BKCa(α subunit)- caveolin-1 complex and BKCa(α subunit)- caveolin-2 complex in myometrium were significantly higher than that of control group(P0.05).. ⑥ Both groups, BKCa(α subunit)- caveolin-1 complex level and BKCa(α subunit)- caveolin-2 complex level in myometrium was negatively related to the level of myometrium contraction. Conclusions ① The uterine smooth muscle is a kind of single-unit smooth muscle with property of autorhythmicity, which make it possible the automatic contraction be detected in vitro. The application of muscle isometric tension experiment in the study of myometrium contraction is feasible. ② Increased BKCa (α, β subunit) expression in myometrium may be strongly correlated with the reduction of myometrium tension. ③ Increased BKCa (α, β subunit) expression in myometrium may be involved in the pathogenesis of PPH caused by uterine atony. ④ There exists BKCa(α subunit), caveolin-1 and caveolin-2 in uterine smooth muscle cells, BKCa(α subunit) and caveolin-1, BKCa(α subunit) and caveolin-2 co-locates on part of the myometrial smooth muscle cell, suggesting caveolins involve in regulating the expression of BKCa. ⑤ Increased expression of BKCa(α subunit)- caveolin-1 complex and BKCa(α subunit)- caveolin-2 comlex in myometrium may be strongly correlated with the reduction of myometrium tension. ⑥ Caveolins