电针对脑缺血再灌注大鼠大脑皮层超微结构的影响.PDFVIP

　2009 6 　34 　3 ·167 · 沈梅红　李忠仁　项晓人　牛文民 (南京中医药大学第二临床医学院, 南京210029 ) 【　】　:探讨电针对局灶性脑缺血再灌注大鼠大脑皮层组 的神经保护作用。:将SD 大鼠随 机分为假手术组、模型组、电针组, 每组各5 只。采用改良Longa 线栓大脑中动脉法复制局灶性脑缺血再灌 注模型。 电针组取穴“百会”及“大椎”, 采用疏密波刺激 30 min, 电流强度1 ～3 m A,频率20 H z/80 H z, 疏、 密波交替时间各为 1.5 s 。用透射电子显微镜观察受损局灶大脑皮层锥体细胞、星形胶质细胞及血脑屏障 等超微结构。:假手术组大鼠大脑皮层超微结构未见病理改变;而模型组大鼠脑组 神经元细胞结构严 重破坏, 线粒体肿胀,嵴断裂, 毛细血管内皮肿胀, 管腔挛缩,甚至闭塞,胶质细胞足突肿胀;经电针处理后脑 组 超微结构损伤变小或基本正常,受损局灶神经元病理结构得到改善。:电针通过影响缺血再灌注局 灶的神经元超微结构从而改善了缺血病灶区域的能量供应及代谢功能, 发挥了对神经功能的保护作用。 【】　脑缺血再灌注　电针　大脑超微结构　大脑皮层神经元 + 【】R 245.9 7 　【】A 　【】1000-0607 (2009 )03-0167-04 Effect of ElectroacupunctureonCerebral Cortex Ultrastructurein Rats with Cerebral Ischemia- reperfusion Injury S HEN M ei-hong, 　L I Z hong-en, 　XIA NG Xiao-en, 　NI U Wen-min (S econd C lin ica l Co lleg e of N anj ing University of Chin ese Med icine, N anj ing 210029 , Ch ina) 【ABSTRACT】　Objective　Toobservetheeffect ofelectroacupuncture (EA)of“Baihui” (GV20)and“Dazhui” (GV 14)on the ultrastructure of cerebral cortex inrats withcerebral ische ia reperfusion (CI/R)injury. ethods　FifteenSD ratswere e- qually rando ized intosha -operation (sha ), odeland EA groups.CI/R odelwas establishedby right iddlecerebral artery occlusionfor 1 hand reperfusionfor 24 h.EA (20 Hz/80 Hz, 1-3 A)was applied to“Baihui” (GV20)and“Dazhui” (GV 14) for30 in.The ische ic cerebral cortextissuewastaken, fixed in2.5%glutaral solution, e bedded inaraldite, cut intosections and stained for observing the ultrastructure changes of cortical pyra idal cells, astrocyte and blood brain barrier (BBB)under trans issionelectronic icroscope.Results　Insha group, the anato ical structureof thecerebral pyra idal cellswasnor al basically.In odel group, theneuronal itochondria and thecapillary endotheliu and the processes of the astrocytegot swell- ing, the itochondrial cristae were broken, th