基于氢化原子荧光法的苹果中Se形态分析研究-井冈山大学学报.PDFVIP

第36 卷第5 期 Vol.36 No.5 冈山大学学报( 自然科学版) 2015 年9 月 Sep. 2015 Journal of Jinggangshan University (Natural Science) 33 冈山大学学报( 自然科学版) 33 章编号：1674-8085(20 15)05-0033-04 基于氢化原子荧光法的苹果中Se 形态分析研究 * 黄田富，胡志彪，刘金仙 （龙岩学院化学与材料学院，福建，龙岩 364000 ） 摘 要：采用连续浸提分离技术，将苹果中的Se 以水溶态、有机物结合态和残渣态 3 种形态选择性地提取， 并用HG-AFS 法检测了各形态的硒含量和总硒含量。结果表明：该方法硒的检出限为0.028 ng/mL ，线性范围 为0~ 10 ng/mL，精密度为2.8%，回收率为94.15%~ 108.95% ；苹果中硒的3 种形态含量不同，水溶态 有机 结合态 残渣态，三者的加和略小于总硒含量，误差在2.6% 以内。 关键词：氢化物发生–原子荧光光谱法；连续浸提；Se 形态 中图分类号：O613.52 献标识码：A DOI:10.3969/j .issn.1674-8085.2015.05.007 SPECIATION ANALYSIS OF SELENIUM IN APPLE BY HYDRIDE GENERATION-ATOMIC FLUORESCENCE SPECTROMETRY HUANG Tian-fu , *HU Zhi-biao, LIU Jin-xian (College of Chemistry and Material Science, Longyan University, Longyan, Fuji an 364000, China) Abstract: Selenium in apple was divided into three fractions: ( 1) water soluble, (2) organic compound, (3) residual by using sequential extraction separation technology, all the three forms of selenium and total selenium were determined with HG-AFS. The experimental results indicated that detection limit of selenium was 0.028 ng/mL, the linearity range was 0 ~ 10 ng/mL, the precision was 2.81%, and recovery percent was 94.15 ~ 108.95%. The content of three forms of selenium in apple were different, water soluble organic compound residual, and the sum of three was slightly smaller than total selenium, the error was less than 2.6%. Key words: HG-AFS; sequential extraction; selenium speciation 硒具有调节维生素A 、C、E 和K 的吸收与利 人体中的硒来源于食品。苹果富含硒，