《香蕉枯萎病产几丁质酶生防菌的筛选与鉴定》》-毕业论文.doc

香蕉枯萎病产几丁质酶生防菌的筛选与鉴定 1 - 海 南 大 学 毕 业 论 文（设计） 题 目：香蕉枯萎病产几丁质酶生防菌的筛选与鉴定 学 号： 姓 名： 年 级： 学 院： 系 别： 专 业： 指导教师： 完成日期： — 年 —月 —日 摘 要 本实验筛选和鉴定出具有较强活性的、对香蕉枯萎病病原菌有一定抑制作用的产几丁质酶的生防菌。从病土中分离得到的菌株92株，通过几丁质培养基的筛选，得到具有产香蕉枯萎病几丁质酶活性的菌株22株。将这22株菌株与香蕉枯萎病病原菌进行拮抗活性测定，通过对峙实验的效果比对和数据分析从中筛选出拮抗效果最强的7株菌株，分别为G-1、G-9、T-6、G-4、T-4、T-9、G-7，对这7株菌株进行测序和Blast比对分析，确定了这7株菌株分别为：Uncultured achromobacter、Achromobacter xylosoxidans、Bacillus amyloliquefaciens、Corynespora cassiicola、Corynespora cassiicola、Corynespora cassiicola和Corynespora cassiicola。 关键词 香蕉枯萎病菌；生防菌筛选；几丁质酶；分子鉴定 Abstract The screening and identification of strong activity of Banana Fusarium wilt, pathogen has certain inhibition of Biocontrol Bacterium Producing chitinase. From the diseased soil in the strain was isolated in 92 strains, by chitin medium screening, with production of Banana Fusarium wilt chitinase activity strains from 22strains of. The22 strains of Banana Fusarium wilt pathogens were antagonistic activity determination, confrontation between the experimental results comparison and analysis of data from the screened antagonistic effect was strongest among7 isolates, respectively, G-1, G-9, T-6, G-4, T-4, T-9, G-7, on which7 strains were sequenced and Blast analysis, determine which of the 7strains were: Uncultured achromobacter，Achromobacter xylosoxidans，Bacillus amyloliquefaciens，Corynespora cassiicola，Corynespora cassiicola，Corynespora cassiicola and Corynespora cassiicola. Keywords：Banana Fusarium Wilt ；Screening of biocontrol bacteria；Chitinase；Molecular identification 目 录 TOC \o 1-3 \p \h \z \u HYPERLINK \l _Toc323724743 1前言 ……………………………………………………………………… PAGEREF _Toc323724743 \h - 5 - HYPERLINK \l _Toc