基础肿瘤理论与实践 瘤凋亡-2015.pptVIP

I decided to look for biochemical alterations that would distinguish normal T cells and preapoptotic T lymphocytes. pH, cation concentrations, membrane permeability, lysosome change and so on. DiOC6, a dye that indirectly measures the mitochondrial transmembrane potential(JEM,1995) Loss of MTP marked a irreversible early step of the apoptotic process(JEM,1995) A cell free system, in which we confronted purified mitochondrial with purifed nuclei. Upon induction of permeability transbility transition, we found that mitochondria released an apoptosis inducing factor that cause chromatin condensa