最新精品Ⅳ型胶原酶心包经上预处理对内关穴注射胰岛素后降糖作用及GLUT4表达分布的影响.docVIP

[最新精品]Ⅳ型胶原酶心包经上预处理对“内关”穴注射胰岛素后降糖作用及GLUT4表达分布的影响 更多专业、稀缺文档请访问——搜索此文档，访问上传用户主页～ ?型胶原酶心包经上预处理对“内关”穴注射胰岛素后降糖作用及 GLUT4表达分布的影响 ?型胶原酶心包经上预处理对“内关”穴注射胰岛素后降糖作用及 GLUT4表达分布的影响 (南通医学院药理学教研室,江苏226001) [摘要]目的:研究?型胶原酶心包经上预处理对小鼠“内关”穴 注射胰岛素后降糖作用及GLUT4表达、分布的影响。, 方法:小鼠“内关”穴上方5mm心包经上注射?型胶原酶,30min 后,“内关”穴注射胰岛素,间隔5min后测血糖,取骨骼肌用 Northernblot方法观察GLUT4mRNA表达,免疫组化法研究GLUT4分布, 并与心包经旁预处理组、蒸馏水心包经上预处理组相比较。, 结果:?型胶原酶心包经上预处理几乎完全阻断“内关”穴注射 胰岛素的降糖效应,其对骨骼肌GLUT4mRNA表达无影响,但可使GLUT4 从胞内储存部位向细胞膜转运减少。, 结论:?型胶原蛋白参与了从胰岛素穴位注射到GLUT4再分布进 而产生快速降糖作用之间的信息传递。, [主题词]梭菌胶原酶/投药和剂量;心胞经;穴,内关;血糖;胰岛 素/投药和剂量 EffectsofPretreatmentofCollagenase?inPericardiumChannelon DecreaseofPlasmaGlucoseLevelandExpressionandDistributi onofGLUT4afterInjectionofInsulininto“Neiguan” PointQianDongsheng,ZhuYifang,LuoLin,etal(Departmentof Pharmacology,NantongMedicalCollege,Jiangsu226001) [Abstract]PurposeToobservetheeffectofpret reamentofcollagenase?inthePericardiumChannelondecreaseofplasmaglucosel evel,expressionofGLUT4mRNAanddistributionofGLUT4a 更多专业、稀缺文档请访问——搜索此文档，访问上传用户主页～ fterinjectionofinsulininto“Neiguan” (PC6).MethodsCollagenase?wasinjectedintothePericar diumChannel5mmabove“Neiguan”(PC6)inthemouse,andinsulinwasinjectedinto “Neiguan”(PC6)30minuteslater.After5minutes,plasmaglucoselevelwasdete rmined.Meanwhile,expressionofGLUT4mRN AanddistributionofGLUT4inskeletalmuscleswereinvesti gatedwithNorthernblotanalysisandimmunohistochemicalmethod.ResultsP retreatmentofcollagenase?inthePericardium Channelalmostentirelyblockedtheeffectofreductiono fplasmaglucoselevelduetoins ulininjectioninto“Neiguan”point.Althoughitdidnotaffect theexpressionofGLUT4mRNA,theGLUT4incytoplasmicmembranewasdecr easedmarkedly.ConclusionCo llagen?mayparticipateinthemessagetransmissionfrominsulini njectiontotherapiddecreaseofplasmaglucoselevel causedbyredistributionofGLUT4., [Keywords]ClostrdiumHistolyticumCollagenase/admin; PericardiumMeridian;PointPC6(Neiguan);BloodGlucose;Insulin/admin, 国家自然科学基金资助项目经穴现象是客观存在的, 但是其实质尚不清楚。胶原蛋白为细胞外间质的主要组成成