粒细胞西集落刺激因子对大鼠薄型子宫内膜治疗的初步研究-妇产科学专业毕业论文.docxVIP

优秀毕业论文 精品参考文献资料 硕 士 学 位 论 文 英 文 摘 要 A B S T R A C T O B J E C T I V E T o investig ate th e effect an d p otential m ech an ism o f g ran u lo cyte colony -stim u latin g factor (G -C S F ) on th in en do m etriu m m od el in itially . M E T H O D 36 sex u ally m ature fem ale v irg in al S p rag ue D aw ley (SD ) rats w ith n orm al estrou s cy cles w ere selected , th in en dom etrium m o dels w ere estab lish ed by p erfusin g anh y drou s alcoh o l into uterin e cav ities for 5 m in utes, th en th ey w ere ran d om ly d iv id ed into fou r grou p s:(l)A cute end om etrial inju ry + G -C S F gro up ：G -C S F (4 0 fig/k g/d ) w as injected sub cu tan eou sly after th e m od el com p leted ev ery 2 4 h o urs for 5 d ay s. (2 ) A cute en dom etrial inju ry + contro l group : sam e do se p hy siolog ical salin e w as injected su b cutan eo u sly after th e m o del com p leted ev ery 2 4 h o u rs for 5 d ay s. (3 )C hron ic en dom etrial injury + G -C S F g rou p : G -C S F ( 4 0 |ag/kg /d ) w as injected sub cutan eou sly three estro us cy cles after th e m o d el com p leted ev ery 24 ho urs for 5 d ay s. (4 )C h ron ic en dom etrial m inju ry + control g roup ： sam e d ose phy sio log ical salin e w as injected sub cutan eo -u sly three estrou s cy cles after th e m o d el com p leted ev ery 2 4 h ou rs fo r 5 d ay s. A ll rats w ere k illed at th e fo urth estru s sin ce th e injection . T ak in g b ilateral uterin e tissu e for h isto lo gical ex am in ation , w e ob serv ed th eir end om etrialm orp h o lo gy ch an g es and m easured the end om etrialth ickn ess b y h em atoxy lin -eosin stain ing (H E ). A t th e sam e tim e w e detected th eir cytok eratin , v im entin exp ression p er un it en d om etrium area u sing im m u n oh istoch em istry techn ique. R E S U L T S ill  硕