华通氏胶干细胞分离鉴定.pdfVIP

Cardoso et al. BMC Biotechnology 2012, 12:18 /1472-6750/12/18 RESEARCH ARTICLE Open Access Isolation and characterization of Wharton’s jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system 1* 1 1 1 1 1 Tereza C Cardoso , Heitor F Ferrari , Andrea F Garcia , Juliana B Novais , Camila Silva-Frade , Marina C Ferrarezi , Alexandre L Andrade2 and Roberto Gameiro1 Abstract Background: The possibility for isolating bovine mesenchymal multipotent cells (MSCs) from fetal adnexa is an interesting prospect because of the potential for these cells to be used for biotechnological applications. Bone marrow and adipose tissue are the most common sources of MSCs derived from adult animals. However, little knowledge exists about the characteristics of these progenitors cells in the bovine species. Traditionally most cell cultures are developed in two dimensional (2D) environments. In mammalian tissue, cells connect not only to each other, but also support structures called the extracellular matrix (ECM). The three-dimensional (3D) cultures may play a potential role in cell biotechnology, especially in tissue therapy. In this study, bovine-derived umbilical cord Wharton’s jelly (UC-WJ) cells were isolated, characterized and maintained under 3D-free serum condition as an alternative of stem cell source for future cell banking. Results: Bovine-derived UC-WJ cells, collected individually from 5 different umbilical cords sources, were successfully cultured under serum-free conditions and were capable to support 60 consecutive passages using commercial StemlineW mesenchymal stem cells expansion mediu