一株脱硫自养菌的鉴定和脱硫特性研究-微生物学报.PDF

微生物学通报 APR 20, 2009, 36(4): 620~626 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 一株脱硫自养菌的鉴定和脱硫特性研究 1 1 1,2 1,2 1,2* 闫 旭 吴晓雨 陆 新 严 群 阮文权 (1. 214122) (2. 214122) 摘 要: 从无锡市某硫酸厂采集的土样中分离到一株具有脱硫性能的自养菌, 对该细菌的 16S rDNA 序列进行了测定, 并根据 16S rDNA 构建了系统发育树, 结合菌落、细菌形态鉴定菌株为那 不勒斯硫杆菌(Thiobacillus neapolitanus), 命名为TL-1 。同时, 考察了TL-1 菌株在不同温度和pH 条件下的脱硫效率, 并在反应器中以氧化还原电位(ORP)为控制条件, 对菌株的脱硫效果和生成 的单质硫颗粒特性进行了研究, 结果表明, 摇瓶实验中, 30°C 、pH 7.5 为脱硫最佳条件。反应器运 行时, ORP 控制在−370 mV 条件下运行效果最佳, 系统能够维持95%以上的硫化物去除率和85% 以上的单质硫回收率, 随着ORP 的增大, 生成的单质硫颗粒粒径有逐渐增大的趋势。 关键词: 脱硫, 鉴定, 影响因素, ORP, 单质硫颗粒 Identification and Sulfide Removal Characteristics of a Desulfurizing Strain YAN Xu 1 WU Xiao-Yu1 LU Xin1,2 YAN Qun1,2 RUAN Wen-Quan1,2* (1. School of Environment and Civil Engineering Jiangnan University, Wuxi, Jiangsu 214122, China) (2. Key Laboratory of Industrial Biotechnology Ministry of Education Jiangnan University, Wuxi, Jiangsu 214122, China) Abstract: A desulfurizing chemoautotroph strain TL-1 was isolated and further identified in this study. Moreover a phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relative bacteria species. Based on the colony and cell morphology, this strain was identified to be the strain of Thiobacillus neapolitanus. The effect of different temperature and pH on the strain was studied in shake