HPLC实验步骤和方法开发.pptVIP

* All HPLC detectors, regardless of design should provide the functions outlined here. * * A prefect detector design would provide all of the listed features. Unfortunately, no such detector exists and any detector design is forced to make compromises. As we will see throughout the presentation, knowledge of these compromises and the effects of the obtained results allow users to use detectors for their maximum benefit. * Sensitivity is one of the most widely cited detector requirements. Generally speaking, most users demand detector that are highly sensitive to their compounds of interest. Many users describe sensitivity simply as the net response (or signal) of the peak (this may be height count, absorbance units, millivolts, intensity, and others). The true measure of sensitivity is better described as a ratio of the signal intensity divided by the intensity of the baseline noise. * * This slide outlines some of the varoius detector capabilities and would be the first step in detector selection. * * * The diagram show the generic layout of a photo diode array detector. All of the energy from a broad spectrum lamp (or lamps) usually covering both the ultarviolet and visible wavelength range is passed through a flow cell. The light is then split into its component wavelenghts on a grating. A series of diodes measures the light intensity over the entire wavelenght range. The result is two types of data, a traditional chromatogram (time vs absorbance) at any wavelentgh in the operating range, and a spectrum (wavelength vs absorbace) at any given time point. Data collected from a PDA detector is often refered to as 3 dimensional data. * * This slide show a small sampling of the compound classes that are often analyzed using FL detectors. Remember that some compounds have a native fluorescence while some may require either a pre-column or post column derivitization to provide fluorescent species. Carbamate Pesticide = Carbofuran Aflatoxin = Aflatoxin B1