厌氧驯化污泥降解2,4,6-tc点p特性及脱氯微生物群落研究.docx

摘要 摘要 上海师范大学硕士学位论文 时，其浓度和脱氯速度成正比。以甲酸钠、乙酸钠、乳酸钠和葡萄糖作为驯化 厌氧污泥的电子供体，同摩尔加入时，其降解效率依次为：乳酸钠乙酸钠 甲酸钠三葡萄糖，与其提供的电子数成正比。当反应体系中加入等电子当量的乳 酸钠、乙酸钠、甲酸钠时，2，4，6．TCP的降解效率接近。 实验还考察了岛和pH对驯化污泥脱氯的影响，结果发现02会抑制脱氯过 程，导致脱氯停止，证明驯化污泥中的脱氯微生物是严格厌氧菌；脱氯反应历 范围为 150 220mV；最适pH为7．5-8。 关键词：厌氧脱氯；驯化；高通量测序；电子介体 Ⅱ 万方数据 Shanghai Shanghai Normal University Master ofPhilosophy Abstract 2,4，6一TCP Mineralization Promoted by Anaerobic Reductive Dechlorination of Acclimated Sludge and Study on Dechlorination of Microbial Community Abstract Witll the continuous development of modem chemical industry,a large number of toxic organic compounds which is difficult to degrade were discharged in the natural environment．For example，chlorophenols had brought great harm to the ecological environment．Therefore，the means of phenol degradation WaS a concerned problem．Anaerobic biodegradafion was a kiIld of economically and effectively method．To discussed the degradation of 2，4，6一trichlorophenols by means of acclimation anaerobic sludge．It’S also contained high-throughput sequencing which combined with experiment results．Its actual applicated for further removed mechanism of chlorine and accelcrated t11e reducfive dechlorination method．The following results were obtained in this paper． ne results showed that it used sodium lactate as electron donor．Anaerobic sludge would be acclimated in a closed container．It could be proved that the domestication process Can enrich the anaerobic bacteria and the sludge had the characteristics of rapid reduction and removed chlorine．Besides，acclimated sludge had higher removal efficiency of ortho chlorine，and it showed obvious substrate specificity．It’S showed that the intermediate production of 2,4，6一trichlorophenols were 2,4一DCP、4-CP、phen01．Because ofthe output of less 2．4一DCP,there could be exist 2 metabolic pathways：The presence of 2，4，6一TCP inferred while removing two ortho chlorine passing．M