反油酸诱导人脐静脉内皮细胞转录组学及生物信息学分析-食品科学.PDF

※生物工程 食品科学 2018, Vol.39, No.22 147 反油酸诱导人脐静脉内皮细胞转录组学及 生物信息学分析 邱 斌，孙文佳，刘 玮，徐同成，刘丽娜，宗爱珍，贾 敏，杜方岭* （山东省农业科学院农产品研究所，山东省农产品精深加工技术重点实验室， 农业部新食品资源加工重点实验室，山东 济南 250100 ） 摘  要：以反油酸诱导人脐静脉内皮细胞后，结合转录组学分析及其生物信息学分析，在转录水平上对相关差异表 达基因进行研究。结果表明：共检测到95 条基因显著变化，其中31 条基因上调，64 条基因下调。并通过生物信息 学分析，其中参与生物进程显著性差异表达基因共772 条，关于细胞组成的基因显著表达性差异基因共46 条，关于 分子功能的基因显著表达性差异基因共122 条。共检测到93 条信号通路，其中显著变化的有11 条，其中包括萜类 骨架生物合成通路、类固醇生物合成通路和脂肪酸代谢通路等。根据蛋白质基因互作通路图，重点分析了同时参与 不同通路的靶基因，包括脂肪酸合成酶、STAT4转录因子及IL-23细胞因子等。 关键词：反式脂肪酸；反油酸；转录组学；信号通路；生物信息学 Transcriptomic and Bioinformatic Analyses of Human Umbilical Vein Endothelial Cells Induced by Elaidic Acid * QIU Bin, SUN Wenjia, LIU Wei, XU Tongcheng, LIU Lina, ZONG Aizhen, JIA Min, DU Fangling (Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China) Abstract: After being induced by elaidic acid, the differentially expressed genes in human umbilical vein endothelial cells were studied by transcriptional analysis and bioinformatics analysis at the transcriptional level. The results showed that a total of 95 genes were found to significantly changed, 31 of which were up-regulated while 64 were down-regulated. The bioinformatics analysis showed that a total of 772 genes were significantly differentially expressed during the biological evolution. There were 46 and 122 differentially expressed genes