烧伤后一氧化氮促进表皮干细胞迁移的作用及机制研究外科学烧伤专业论文.docxVIP

英文缩写词表缩写 英文缩写词表 缩写 英文名称 中文名称 NO Nitric Oxide 一氧化氮 SNAP S-nitroso·-N--acetylpenicillamine S一亚硝基．N．乙酰一DL．青霉胺 hESC Human Epidermal stem cell 人表皮干细胞 ESCs Epidermal stem cells 表皮干细胞 rpm Rotation per minute 每分钟转速 mln Minute 分 hr Hour 小时 L—Arg L—arginine L一精氨酸 FCM Flow Cytometric 流式细胞术 FBS Fetal bovine serum 胎牛血清 K．SFM Keratinocyte SFM 无血清角质细胞培养基 PVDF polyvinylidene fluoride 聚偏二氟乙烯 EGF human epidermal growth factor 表皮生长因子 LRC Label—retaining cell 标记滞留细胞 siRNA Small interfering RNA 小干扰RNA BPE Bovine Pituitary Extract 牛垂体提取物 WB Western Blotting 蛋白质转移吸印技术 Gly Glycine 甘氨酸 TBSA Total body surface area 总体表面积 0D Optical density 光密度 FITC Fluoresecence isothiocyanate 异硫氰酸荧光素 HE Hematoxylin-eosin 苏木精．伊红 DAB 3,3．diaminobenzidine 二氨基联苯胺 K19 Keratin 19 角蛋白19 BrdU 5-Bromo--2··deoxyUridine 5一溴脱氧尿嘧啶核苷 CCK．8 Cell Counting Kit一8 细胞计数试剂8 L—NMMA NG-Monomethyl．．L．．arginine N．甲基一L一精氨酸 万方数据 第三军医大学博士学位论文Nitric 第三军医大学博士学位论文 Nitric oxide accelerates wound healing by promoting epidermal stem cell migration via cGMP·-Rho GTPase signaling Abstract Wound healing is a complex process mediated by many different factors responsible for the regeneration and reorganization of wound toward its normal architecture．The mechanisms of skin repair has not been completely elucidated，and many cellular components contribute to maintenance skin homeostasis and regeneration．Epidermal stem cells that reside in the basal layer of the skin epidermis and hair follicles bulge are essential for skin homeostasis and wound healing．Under normal circumstances，the homeostasis of epidermis and hair follicles is maintained by their stem cells．However，both stem cell populations are capable of regenerating the two structures，upon skin wounding．Through fate—mapping experiments，ESCs were shown to be rebuild to the epidermis and migrate in a linear manner toward the center of the wound．However,little is known regarding the mechanisms that stimulate ESCs migration during wou