核因子Y的C亚基(NF-YC)与大鼠脑缺血再灌注损伤关系的分析.pdfVIP

中文摘要 核因子Y 的C 亚基 （NF -YC ）与大鼠脑缺血再灌注损伤关系的研究 活力则明显提高，NF-YC 的siRNA 起到细胞保护作用，说明NF-YC 在脑缺血再灌注 损伤后起到促进神经元凋亡的作用。 结论： 1、大鼠脑缺血再灌注后NF-YC 的表达变化与Bim 和caspase-3 的表达变化一致， 均呈先增加后降低的趋势，且共定位于神经元细胞中。 2 、在大鼠脑缺血再灌注后，NF-YC 的高表达可以通过Bim 的介导促进神经元细 胞凋亡。 关键词：核因子Y 的C 亚基 （NF-YC ），Bim，脑缺血再灌注损伤，神经元凋亡， 氧糖剥夺 作 者：王云锋 指导老师：崔 岗 核因子Y 的C 亚基 （NF -YC ）与大鼠脑缺血再灌注损伤关系的研究 英文摘要 The relationship between the subunit C of nuclear factor Y (NF-YC) and focal cerebral ischemia-reperfusion injury in rats Abstract Objective: To study the role of the subunit C of nuclear factor Y (NF-YC) in rats’ brain following ischemia-reperfusion injury, and further research the mechanism of NF-YC in cerebral ischemia reperfusion at a cellular level. Methods: Healthy male Sprague-Dawley rats were randomly divided into 3 groups: operation group, sham operation group and control group. The operation group was established with the cerebral ischemia-reperfusion injury model by inserting thread into middle cerebral artery to occlude it. The expression of NF-YC, Bim and caspase-3 in rat hippocampus were measured by western blot. Immunochemistrical analysis verified the expression of NF-YC. We used immunofluorescence method analyzing their positions in the tissue of rat hippocampal cells. And TUNEL detected ischemic hippocampus CA1 area neurons apoptosis. Then, in the oxygen-glucose deprivation- induced PC12 cells, we made use of siRNA-NF-YC to interfere the expression of NF-YC, respectively using western blot and CCK-8 method to study NF-YC role in cell apoptosis. Results: We found tha