《SNT1204-2003-植物及其加工产品中转基因成分实时荧光PCR定性检验方法》.pdfVIP

Protocol of the real-time PCR for detecting genetically modified plants and their derived products T9JMfMfSiSflF**a 3. 1 AmpErase UNC 89 1 uracil N-glycosylase. 3 . 2 C,@ : C : c y c l e , t : t h r e s h o l d , S + E ~ W XBrl%%B~Bl%~Brl~f$BifEM!!5BrlBCIS~. 3 . 3 1PC:internal positive control, #me 3 . 4 NTC: no template control, 9fiMR. 3 . 5 PCR: polymerase chain reaction, m$$PCR. 3.6 DNA deoxyribonucleic acid, li#N@#@. 3.7 dNTP a deoxyribonucleoside triphosphate, li#N% =@@ 3 . 8 dATP: deoxyadenosine triphosphate,jl#NJBW%E%O. 3.9 dCTP8deoxycytidine triphosphate,B!Nl@BZ@@* 3. 10 dG?P,deoxyguanosine triphosphate,ENgl$%Z@. 3. 1 1 dUTPrdeoxyuridine triphosphate,j@,#91%jZ%OBgp. 3. 12 bpgbase pair,MBN. 3.13 ~a~ maDNA fiCe@. 3. 14 Tris: tris(hydroxymethy1) aminomethane,.Z(E2:! Ffr)aS:!Sm 3. 15 T E I T ~ ~ ~ - C ~ , E D T A ~ # ~ ~ 3. 16 Lectintl#B#€#. 3.17 ZEIN:#@B#EB. 3. 18 P ~ t ~ ~ P c apsheo :s p h o e n i l p y r u v a t e c a r b o x y l a s e , ~ @ ~ ~ ~ ~ ~ ~ l f 1 3 P B ~ ~ . 3. 19 tRNALeu:#W8#HB * 3.20 18s rRNA:@@ 18 s @@# RNA B$. 3.21 CaMV 35S:35S promoter from cauliflower mosaic virus,% El TZ#%ZiV%3i$H3 5 S G 3 F e 3.22 NOS: terminator of nopaline synthase gene ,MlB@igiff$tsl)SEl%kF. 3.23 FMV35SZ35Spromoter from a modified figwort mosaic virus(cau1imovirus group) ,Z#ZUj-J$j 33 35S!aS!JT. 3.24 NPTII :neomycin-3-phosphotransferase gene, %~~-3-@@$$ffl% H . t 3.25 BAR :phosphinothricin acetyltransferase gene from Bacillus amylolique faciens ,S/E 8T f- Bacillus amylolique faciens f T R ZB%$$%hBA2H0 3.26 P A T *phosphinothricin acetyltransferase gene, *Tf# 2m$$bAgm. 3.27 GOX! glyphosate oxidoreductase gene, fgB.$IBEmSH a 3.28 CryIA(b): a synthetic gene encodes the first 648 amino acids, insecticidal-active ftruncated product identical to that of cr