金钗石斛的茎段组织培养与植株再生.PDF

　 　 2002 33 11 Chin s Traditional and H rbal Drugs · 1031· 高培元,陈健妙,甘　铨 (, 　570216) 　: 　。　; 、。 　 M S 6-BA ( 0. 5 mg /L)、 NAA ( 0. 2 mg /L) , 6-BA ( 3. 0 mg /L)、 NAA ( 0. 5 mg / L) , IBA ( 0. 3 mg /L)、 NAA ( 0. 1 / ) ( 95% )。、、 ( 1∶1∶1) mg L 。 　。 : ;; : R282. 13　　　: A　　　: 0253 2670( 2002) 11 1031 03 Tissue culture of stem segment and plantlet regeneration of D endrobium nobile GAO P i-yuan, C HEN Jian-miao, GAN Quan ( Hainan Tissu Cultur R s arch C nt r for Tropical Plant, Haikou 570216, China) : 　 Abstract Object To sp d up th artificial propagation of th costly m dicinal plant Dendrobium no- . 　 bile Lindl Methods Th t nd r st m s with lat ral buds w r tri d as th xplants and cultur d on various . 　 cultur m dia with diff r nt portions of hormon Results Th b st m dium for bud induction w as th M S basic m dium w ith addition of 6-BA ( 0. 5 mg /L) , N AA ( 0. 2 mg /L) , whil M S with th addition of 6-BA ( 3. 0 mg /L) N AA ( 0. 5 mg /L) , w as suitabl for propagation, M S with addition of IBA ( 0. 3 mg /L) , N A A ( 0. 1mg /L) w as b tt r for root growing , th striking grow th rat w as up to 95% . Th young sprout w as cultur d fin on th bas mat rial mix d with coco crumbs, volcanic rocks and brick fragm nts ( 1∶1∶1) and cov r d by dri d pt ridophyt . Conclusion　 To provid th ff ctiv w ays to r s rv th r - sourc s of D . nobil e and last its utlization. Key words: Dendrobium nobile Lindl. ; tissu cultur ; plantl t r g n ration 　　 D endrobium nobile Lindl. ,。 ,,, ,, ( d ndrobin )、 ( nobilonin )、 6- 。