diz_色谱柱的故障诊断与维护2.ppt

* * * To summarize this section on troubleshooting, most HPLC column problems can be prevented provided the proper precautions are taken. Read the list... and remember to troubleshoot baseline changes. These are an early indication that there may be a column problem. Thank you for your time today! Are there any questions? 6M盐酸胍、异硫氰酸胍、尿素等溶液可用于清除蛋白质的沉积 XDB, 有机聚合物等填料可使用高pH 水溶液 /有机溶液，如： 0.01M NaOH 50% 异丙醇 /水溶液，硅胶基质应尽量减少接触时间 (15-30 min). 色谱柱的修复和再生 -- 化学变化 -II Example: 4.6 mm ID x 15 cm Zorbax 300SB-C18 Flow rate: 1 mL / min; Temp: ~80℃ A: 0.1% TFA in water B: 0.1% TFA in acetonitrile 100% A for 30 min.; 0-100% B over 30 min.; Hold 100% B for 30 min., Return to 100% A in 5 min. Repeat 3 times. 总 结 如果使用时加以注意，大部分的液相色谱柱的问题是可以预防的 - 流动相必须是互溶的，无颗粒 - 使用保护柱和在线过滤器，并定期更换 - 使用恰当的样品制备方法 - 使用恰当的色谱柱清洗程序 - 不同类型的色谱柱，请选择不同的操作条件 (如： SB: pH 1-6, XDB: pH 3-11) - 保存柱反压及重要的色谱参数的记录 (如： R, N, As, k) - 色谱柱需保存在有机溶剂中 (乙腈) * Hi There... * We will start with some more survey results from LC/GC to show some of the most common problems with columns and HPLC. The number on problem by far, is high back pressure. This can be caused by plugged frits as well as column contamination or solvent/sample immiscibility. Poor reproducibility, low sample recovery, and loss of resolution are the next most common problems as reported by chromatographers. Instability and leaks are usually a function of the HPLC hardware and are next as well as column voids which can be caused by the equipment as well. pH range, low plate count and column overload are all column related problems due to a deteriorating phase or poor phase stability. Cost is also reported as a column problem, and miscellaneous comprises 15%. * The outline of this section will be to first cover some troubleshooting of the HPLC system and column problems. We will look at chromatographic baseline symptoms and solutions. We will talk about a troubleshooting guide. We will then move into a discussion