正交设计实验在丹参提中的应用.docVIP

第 PAGE 16 页 正交试验设计在丹参提取中的应用 摘要 丹参是一种具有广泛的药理活性和应用价值的常用中药材，丹参化学成分的提取工艺研究较多，本文探讨采用正交试验设计提取丹参酮的优选工艺。目的:探讨正交试验设计在丹参提取工艺中的应用。方法:采用L9(34)正交试验设计，以乙醇浓度、提取时间、提取次数及溶剂用量作为影响为因素，分辨考察丹参中脂溶性和水溶性成分提取情况，脂溶性成分以丹参酮ⅡA的含量为指标，水溶性成分以原儿茶醛含量为指标，确定最佳提取条件和影响因素。结果：丹参提取的最佳工艺为8倍量乙醇，提取1次，每次提取1.5h。结论：丹参药材中有效成分包括水溶性成分和脂溶性成分两大类，综合两类成分作为优选丹参提取工艺指标，比较全面可靠。 关键词：正交试验设计；丹参；提取工艺 Abstract: Salvia is a broad pharmacological activity and application value commonly used herbs.Objective:To investigate the orthogonal experimental design in Salvia extraction application. Methods: L9(34) orthogonal design to ethanol concentration, extraction time, extraction times and solvent consumption as the impact of factors distinguish investigated Salvia extract fat-soluble and water-soluble components of the situation, fat-soluble ingredients to the content of tanshinone ⅡA as an indicator, water-soluble components to protocatechuic aldehyde content as an indicator to determine the optimum extraction conditions and influencing factors. Results: The optimum extraction Salvia is 8 times the amount of ethanol extract a times, each extraction 1.5h. Conclusion: The active ingredient in Salvia miltiorrhiza water-soluble components and fat-soluble ingredients include two categories, integrated two types of components as a preferred Salvia extraction process indicators, more comprehensive and reliable. 目 录 TOC \o "1-3" \h \u HYPERLINK \l "_Toc358812527" 引言 4 HYPERLINK \l "_Toc358812528" 1 正交试验设计 PAGEREF _Toc358812528 \h 4 HYPERLINK \l "_Toc358812529" 1.1 正交试验设计原理 PAGEREF _Toc358812529 \h 5 HYPERLINK \l "_Toc358812530" 1.2 正交试验设计法中的几个常用名词 PAGEREF _Toc358812530 \h 5 HYPERLINK \l "_Toc358812531" 1.3 正交设计试验方法 PAGEREF _Toc358812531 \h 6 HYPERLINK \l "_Toc358812532" 1.4 正交试验设计的主要用途 PAGEREF _Toc358812532 \h 7 HYPERLINK \l "_Toc358812533" 2.2 实验内容 PAGEREF _Toc358812533 \h 9 HYPERLINK \l "_Toc358812534" 2.2.1 仪器与试药 PAGEREF _Toc358812