in accumbal dopamine in the rat are partly mediated via the资料.pdfVIP

Dexmedetomidine-Induced Decreases in Accumbal Dopamine in the Rat Are Partly Mediated via the Locus Coeruleus Robert A. Whittington, MD, and La´szlo´ Vira´g, MS Department of Anesthesiology, College of Physicians and Surgeons, Columbia University, New York, New York We have demonstrated previously that the systemic ad- liquid chromatography coupled to electrochemical de- ministration of the selective 2-adrenoceptor agonist tection. Dex produced significant decreases in extracel- dexmedetomidine (Dex) decreases extracellular dopa- lular DA in the NAcc. Accumbal DA decreased maxi- mine (DA) levels in the rat nucleus accumbens (NAcc). mally to 68.9% 8.8%, 75.1% 6.5%, and 77.04% Because the locus ceruleus (LC) is a noradrenergic cen- 12.8% of baseline in the Dex-High, Dex-Mid, and Dex- ter linked to several of the pharmacological effects of Low groups, respectively. No significant decrease in Dex, we investigated the role of the LC in Dex-induced extracellular DA was observed in the control group. modulation of accumbal DA. Microdialysis probes The coadministration of the highly selective 2- were implanted in the NAcc and LC of Sprague- adrenoceptor antagonist (n 6) RS 79948 20 mM pre- Dawley rats, and Dex 5 mM (Dex-High, n 6), Dex 0.5 vented the Dex-induced decrease in accumbal DA. mM (Dex-Mid, n 5), Dex 5 M (Dex-Low, n 6), or These data suggest that the LC plays a role in Dex- artificial cerebrospinal fluid (control, n 5) was admin- induced modulation of mesolimbic DA and support the istered in the LC via retrograde microdialysis for 45 hypothesis that noradrenergic systems can regulate re- min. Extracellular DA levels were continuously mea