纳米二氧化锡电极的制备及其用于水体中大肠杆菌的快速计数研究.PDF

2005 年第 63 卷 化 学 学 报 Vol. 63, 2005 第 14 期, 1313～1317 ACTA CHIMICA SINICA No. 14, 1313～1317 ·研究论文· 纳米二氧化锡电极的制备及其用于水体中大肠杆菌的快速计数研究 * 张 文 唐 辉 顾 静 耿 萍 金利通 (华东师范大学化学系 上海 200062) 摘要 制备了新型纳米二氧化锡电极, 将此电极用于水体中大肠杆菌的快速计数研究. 实验结果表明, 用该电极为工 作电极, 采用计时电流法能简便、快速、灵敏地对水体中的大肠杆菌进行计数. 通过大肠杆菌脂质过氧化后丙二醛含 量的检测, 对大肠杆菌在纳米二氧化锡电极上的电化学响应机理进行了初步的探讨. 关键词 纳米二氧化锡电极; 大肠杆菌; 快速计数; 脂质过氧化; 计时电流法 Study on Preparation of Nano-SnO Electrode and Its Application to 2 the Fast Counting of E. coli in Water ZHANG, Wen TANG, Hui GU, Jing GENG, Ping JIN, Li-Tong* (Department of Chemistry , East China Normal University, Shanghai 200062) Abstract A novel nano-SnO electrode was fabricated and applied to the fast counting of E. coli in water. 2 The nano-SnO2 electrode was used as bioelectrochemical sensor in chronoamperometry to count fastly E. coli with the advantages of quickness, simple operation and high sensitivity. Through assaying the MDA content of E. coli solution treated by electrochemical oxidation, the oxidation mechanism of E. coli at nano- SnO2 electrode was investgated and satisfactory results were obtained. Keywords nano-SnO2 electrode; E. coli ; fast counting; lipid peroxide; chronoamperometry 水体的微生物学检验, 特别是肠道细菌的检测, 在 大, 需用 48 h 才能获得结果. 这些技术已经很难满足快 确保饮水安全和控制传染病等方面有着重要的意义. 水 速计数大肠杆菌的需要. 源中大肠杆菌的数量是直接反映水源被人畜排泄物污