乳腺癌基因芯片数据分析-中国科技论文在线.PDF

复里亏提(医学版) Fudan Univ J Med sci 169 乳腺癌基因芯片数据分析 蒋定锋 高 峻 赵耐青 (复旦大学公共卫生学院卫生统计与社会医学教研室 上海 200032) 【摘要】 目的 以乳腺癌病人的表达谱芯片数据为基础，探寻乳腺癌复发的相关基因。方法 对标化芯片数据 进行缺失值处理后，分别用单因素COX回归模型和综合了聚类及多因素COX回归的综合法来筛选兴趣基因， 然后通过兴趣基因对病人做样品聚类，以灵敏度、特异度、约登指数和Kaplan．Meier法评价分类效果，最后结合 文献和蛋白质数据库探寻乳腺癌复发的相关基因。结果 综合法筛出的3O个P0，01的基因对乳腺癌病人复 发状况的预测效果最佳；单因素COX回归筛出的102个P0，01的基因的预测效果较差；单因素C0X回归筛 出的15个P0．001的基因预测效果最差。结论 综合法筛选得到的3O个基因可用来评价病人的预后状况， 为进一步的生物学研究提供待选基因。 【关键词】乳腺癌； 基因芯片； 聚类分析； COX回归模型 【中国图书馆分类法分类号】R 737．9，0 212，1 Microarray Data Analysis for Breast Cancer JIANG Ding—feng，GAO Jun，ZHAO Nai—qing (Department ofHealth Statistics and Social Medicine，Sdmol ofPublic Health，Fudan University Shanghai 200032，Ckina) 【Abstract】Purpose To study the relapse-related genes based on gene expression profiles from breast can． cer patients with different clinical outcomes． Methods Firstly，univariate COX regression model was used to analysis the microarray data tO select the potential genes．Secondly，an integrated method composed of cluster and multivariate O0X analysis was also carried out，Then K—means cluster method was applied tO clas— sify the relapse situation of patients．Sensitivity，specificity，Youden’S index and Kaplan·Meier analysis were used tO evaluate the genes selected by different methods， Results The 30 genes(P0．01)selected by the integrated method performed best when predicting the relapse situation of patients，The 102 genes(P0． 01)by univariate COX analysis performed well while the 15 genes(P0．001)by univariate COX analysis performed worst， Conclusions The 30 genes selected by the integrated method，especially the genes which wer