课题研究合作协议书中华医学会麻醉学分会.doc

PAGE PAGE 8 完全弗式佐剂关节炎大鼠疼痛行为学及脊髓NF-κB的表达 广州医学院第二附属医院麻醉学教研室 万丽1黄焕森1 胡春旭1 高崇荣1 罗爱林2 田玉科2 [摘要] 目的 制备稳定的油包水剂型完全弗式佐剂单关节炎大鼠模型，并对大鼠疼痛行为学及脊髓背角NF-κB表达进行观察和探讨。方法 雌性250～300g SD大鼠24只，随机分为假手术组、完全弗氏佐剂组；以2.8%戊巴比妥钠1ml/kg腹腔注射麻醉后，将制备好的含灭活结核杆菌的粘稠油包水型完全弗式佐剂50μl注射至大鼠右踝关节腔内，假手术组注射50μl生理盐水至大鼠右踝关节腔内，观察大鼠完全弗式佐剂注射前2d，注射后1、4、7、14、21、28d的机械压痛、辐射热痛、关节肿胀周径（内外踝下缘周长）及脊髓背角NF-κB表达的变化。结果 ①大鼠注射完全弗式佐剂3h，右踝关节出现明显肿胀，但局部红、热不明显，注射后24h右踝关节红肿显著且波及足跖面，少数大鼠甚至形成踝足跖部皮肤破损出血，1w时红肿渐局限于右踝关节局部，并持续至注射后4w。②大鼠右踝关节周径（cm）在注射后4d（3.10±0.02）至4w（3.05±0.01）较对侧（2.37±0.01）及注射前（2.37±0.01）显著增加，差异具有显著性，p﹤0.01。③右踝关节注射完全弗式佐剂1w后，大鼠患侧压痛阈值及辐射热痛阈值开始降低，并持续至观察期4w。机械压痛阈值在注射后1w显著下降，压痛阈值为（472.06±85.99）g，与注射前（718.74±101.01）g相比，差异具有显著性，p﹤0.05；辐射热痛阈值在注射后1w显著下降，平均为（5.07±0.41）s，与注射前（7.79±0.49）s相比，差异具有极显著性，p﹤0.01。④完全弗氏佐剂组大鼠患侧腰段脊髓背角Ⅰ～Ⅵ层NF-κB表达明显高于假手术组，差异具有显著统计学意义（p﹤0.01）。结论 关节腔内注射油包水型完全弗式佐剂可获得稳定的单关节炎疼痛模型，大鼠关节致炎后出现明显的辐射热及压痛痛觉过敏，且持续时间长，痛敏阈值稳定，脊髓背角Ⅰ～Ⅵ层NF-κB表达明显升高 [关键词] 佐剂性关节炎；炎性痛； NF-κB；脊髓背角 The algesia behavior and the expression of NF-kappa B in spinal dorsal cord in complete Freurd’s adjuvant arthritis rats. Wan Li, Huang Huan-sen, Hu Chun-xu, et al. Department of Anesthesiology, The Second Affiliated Hospital of Guangzhou Medical College, Guangdong, 510260, China. [Abstract] Objective To explore the expression of NF-kappa B in spinal dorsal horn and investigate the algesia behavior of the anle arhititis after complete Freund’s adjuvant injection in rats.Methods Twenty-four female SD rats were randomly divides into sham group and complete Freund’s adjuvant group. Adjuvant arthritis model was produced by injection of complete Freund’s adjuvant (CFA) 50 μl to the right ankle joint after anesthesia the rat. the same volume saline was injected to the rat right ankle joint in sham group.The mechanical pain threshold, paw withdrawal thermal latency (PWTL) and the diameter of ankle were investigated after CFA injection for 24 hr, 4rd, 1w to 4w. Immunohistochemistry was used to detect the NF-kappa B expression in spinal dorsal horn. Results 3