用离子交换吸附法回收Bt发酵液上清中的增效物质-中国生物防治学报.PDF

( ) 20 1 34 - 37 　　　　　　　中国生物防治 　Chinese Journal of Biological Control 　　　　　　　2004 年 2 月 用离子交换吸附法回收 Bt 发酵液 上清中的增效物质 沈锡辉 , 王开梅 , 张志刚 , 陈在佴 ,镇 达 (湖北省生物农药工程研究中心 , 武汉 　430064) 摘要 : 采用阳离子交换树脂进行了回收Bt 发酵废弃上清液中增效物质的初步研究 ,确定了最 适的离子交换树脂 。其最适回收条件为p H5 ,流速 1. 5 倍 v/ h ,废弃上清液中增效物质的回收 效率可达 83 % 。回收的浓缩上清液与去上清菌浆比例为 16 ∶1 时对棉铃虫毒杀作用最强 ,表 现出最好的增效作用 。 关 　键 　词 : 苏云金芽孢杆菌 ; 增效物质 ; 离子交换吸附; 回收 ; 增效活性 ( ) 中图分类号 : S476 . 11 　　文献标识码 : A 　　文章编号 : 1005926 1 2004 0 1003404 Recovering Potentiator f rom Waste Supernatant in Production Process of B acill us t h u ri ngiensis by IonExchange Adsorption Column Chromatography SHEN Xihui , WAN G Kaimei , ZHAN G Zhigang , CHEN Zaier , ZHEN Da ( Hubei Biop esticide Engineering Research Center , Wuhan 430064 , China) Abstract : Different t ypes of ionexchange resin were used to recover potentiator from waste su pernat ant in t he p roduction p rocess of B acil l us t h u ri ng iens is subsp . k u rs t ak i st rain M P342 . The st atic adsorption showed t hat D00 1 had t he best adsorption ability and also t he best releasing abili t y during elution . The optimized condition for recovering potentiator t hrough ionexchange ad sorption was 1) Adsorption at p H 5 , and 2) Eluting rate at 1. 5 volume of resin/ h . The recovery rate of t he potentiator in t he waste supernat ant was 83 % based on t he inhibition rate against α Escherichia col i D H5 . The concent rated supernat ant showed highest potentiation rate when in corporated wit h nosupernat ant Bt sludge at a ratio of 16 ∶1. Key words : B acil l us t h u ri ng iens is ; potentiator ; ionexchange adsorption ; recovery ; synergism