慢病毒介导的siRNA靶向CD47基因对人喉癌细胞Hep-2增殖、凋亡的.PDF

军 杂 201381388 634 siRNACD47Hep-2 高树峰，张少容，徐莲，李黎，万俊，杨春平，刘建国，汪美群，刘月辉 [] siRNACD47Hep-2 CD47siRNA Hep-2RT- PCRCD47 mRNAWestern blotting CD47MTT CD47-siRNA Hep-2CD47-siRNA Hep-2 RT-PCR Western blotting CD47mRNA (P 0.05)CD47 mRNA76%~82% CD4777% 48h MTT (P 0.01) siRNACD47Hep-2CD47 Hep-2 []CD47 RNAHep-2 []R739.65 []A []0577-7402(2013)08-0634-05 Effect of small interfering RNA targeting CD47 gene mediated by lentivirus vectors on proliferation and apoptosis of human laryngocarcinoma Hep-2 cells * GAO Shu-feng, ZHANG Shao-rong , XU Lian, LI Li, WAN Jun, YANG Chun-ping, LIU Jian-guo, WANG Mei-qun, LIU Yue-hui Department of Otorhinolaryngology, Head and Neck Surgery, Second Ailiated Hospital, Nanchang University, Nanchang 330006, China * Corresponding author, E-mail: zhang_shaorong@ This work was supported by Natural Science Foundation of Jiangxi Province (20114BAB205069) [Abstract]ObjectiveTo study the effect of small interfering RNA (siRNA) targeting CD47 gene mediated by lentivirus vectors on proliferation and apoptosis of human laryngocarcinoma Hep-2 cells. Methods After building lentivirus vector, a siRNA directed against CD47 gene was transfected into Hep-2 cells. Cell morphological changes were observed by fluorescence microscopy. The changes in CD47 mRNA expression were detected by semi-quantitative RT-PCR , and the protein of CD47 was evaluated by Western blotting. Suppression of proliferation and apoptosis of Hep-2 cells were observed by MTT assay. Results After the laryngocarcinoma Hep-2 cells were transfected by CD47-siRNA lentiviral plasmid, it was showed by fluorescence microscopy that the CD47 siRNA was able to effectively suppress the Hep-2 cell proliferation, the cells were deformed and diminished in size obviously, and necrosis and apopto