兔LQT2模型心室肌复极化的性别差异.pdfVIP

Acta Physiologica Sinica , December 25, 2005, 57 (6): 749-754 749 Research Paper Gender-related differences of ventricular repolarization in LQT2 rabbit model * LIU Li-Ping, YANG Lin , ZHAO Zhao, CHEN Qian Department of Cardiology, Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, the First Affiliated Hospital of Medical College, Xi’an Jiaot ong University, Xi’an 710061, China Abstract: To explore the cellular mechanism responsible for the gender-related differences of ventricular tachyarrhythmias in long QT syndromes (LQTS), we observed the characteristics of pre-existing electrophysiological heterogeneity and dynamics of ventricular repolarization in different gender of LQT2 rabbit models. The intracellular floating microelectrodes technique was used to record transmembrane action potentials simultaneously from epicardial and endocardial sites of the arterially perfused rabbit left ventricular wedge preparation; in the mean time, the electrocardiogram (ECG) was also recorded. The wedge preparations were placed in a small tissue bath and arterially perfused with normal Tyrode’s solution (control group), 100 µmol/L dl-sotalol Tyrode’s solution (LQT2 model group), and 100 µmol/L dl-sotalol plus 3.0 mmol/L KCl Tyrode’s solution (LQT2 model plus hypokalemia group), buffered with 95% O2 and 5% CO2 [(36.0±1) ºC]. Double diastolic threshold currents were delivered with basic cycle length (BCL) 2 000, 1 000 and 500 ms (S1), respectively, to record transmembrane action potentials and transmural ECG. Each driving train of S1 is with 20 beats. To determine action potential duration restitution (APDR) curves, the S1-S2 programmed stimuli were used. The tissue was paced at 1 000 ms and 500 ms cycle length (S1) for eight beats, followed by a single premature stimulus (S2). The S1-S2 coupling interval was progressively shortened with 10 ms d