脊尾白虾与三疣梭子蟹微孢子虫病的病原和病理-临床兽医学专业论文.docxVIP

上海海 上海海洋大学硕士学位论文 II II 肌细胞内大量微孢子虫孢子寄生，肌原纤维模糊，大部分消失，细胞核变形，异 染色质浓缩，线粒体嵴断裂，消失。鳃上皮细胞坏死，线粒体水肿、嵴消失，细 胞核核质边缘化，核解体消失，细胞质及细胞器消失，细胞失去典型细胞结构。 胃中微孢子虫寄生导致细胞膜消失，成熟孢子、多数细胞器及细胞质被释放到细 胞外环境中，染色质边聚。肠内细胞膜结构被微孢子虫破坏，细胞核周围溶酶体 数量增加，核浓缩破碎甚至消失，细胞器消失，细胞空泡化。 关键词：脊尾白虾，三疣梭子蟹，微孢子虫，超微结构，SSU rRNA，病理 PAGE PAGE III The Pathogen and Pathology of Microsporidiasis in Ridgetail White Prawn, Exopalaemon carinicaud and Swimming Crab, Portunus trituberculatus ABSTRACT The “muscle whitish disease” occurred in a marine polyculture of ridgetail white prawn, Exopalaemon carinicaud and swimming crab, Portunus trituberculatus in Qidong, Jiangsu province, China. Morphological and structural analysis of pathogens confirmed that they were two different microsporidian by using three microscopic techniques initially. The universal microsporidia primers were used to amplify the SSU rRNA gene and the molecular identifications were conducted further. The pathological analysis of infected E. carinicaud and P. trituberculatus were also performed. The results were as follows: The microsporidan with sporophorous vesicle structure was observed in the prawn E. carinicaud. One sporophorous vesicle containing eight oval-shaped uninucleate spores was measured 5.4±0.55 (4.4-6.6μm), mature and fresh spore was approximately 2.3±0.25 (1.7-2.8μm) ×1.5±0.19 (1.2-1.9μm) with 9-10 polar ?lament coils which aligned irregularly in two ranks and it was 2.0±0.16（1.7-2.3μm）×1.3±0.15（0.96-1.6μm） after fixing. The Morphological characteristics shared similarity with the genus Thelohania. A cloned ribosomal SSU-ITS-LSU (partial) gene fragment of 1873bp was obtained from the microsporidian. Phylogenetic analysis suggested a closest affinity with P. morhaphis. Their sequences identity was 91% but significant differences were found on morphological characteristics. Due to the contradiction between traditional morphological and molecular taxonomic data, this microspridian was placed into the unclassified members as Microsporidium sp. Pat